Effect of HFD and BM on blood-brain barrier permeability and neuroinflammatory markers. Concentrations of Evans blue dye (A) and mRNA expression of IL-22 (B), IL-17R (C), NF-κB1 (D) and IL-16 (E) in brains of mice fed HFD with and without BM. Values represent the mean ± SE (n = 4). Each sample was analyzed in duplicate, in two independent experiments. a,b,cMean values with common letters do not differ (p < 0·05).