Effect of HFD and BM on glial cell activation markers. Figure 2 represents microglial activation markers, Iba1 protein levels (A), Aif1/Iba1 mRNA expression (B) and CD11b mRNA expression (C) as well as astrocyte activation markers, GFAP protein levels (D), GFAP mRNA expression (E) and S100β mRNA expression (F). The bar graph in Figure 2A and 2D represents the densitometry scan of 17-kD Iba1 and 50-kD GFAP proteins, respectively. Values for qRT-PCR represent the mean ± SE (n = 4), while those for western blot analysis represent the mean ± SE (n = 3). Each sample was analyzed in duplicate, in two independent experiments. a,b,cMean values with common letters do not differ (p < 0·05).