Neuronal processes are the tracks for rod microglial trains. Double-labeling of microglia (Iba1; red) with neuronal structural markers (green) and Hoechst nuclear dye (blue). (A) Ramified microglia (Iba1; red) in uninjured sham brain showed no association with MAP-2, neurofilament, or the pan-neuronal marker. Rod microglia in the sensory cortex 7 days post-injury were in close apposition with dendrites (MAP-2) and axons (neurofilament). (B) Additional staining with a pan neuronal marker clearly demonstrated the proximity of trains of rod microglia to neuronal structures via confocal analysis.