Figure 6

Western blot analysis of the CRMP2 protein cross-reacting proteins in sham control and permanent middle cerebral artery occlusion (PMCAO) with or without pituitary adenylate cyclase-activating polypeptide (PACAP) treatment. Proteins cross-reacting with the anti-CRMP2 protein antibody are visible as three constitutively present proteins (approximately 70, 65, and 63 kDa size) in all samples. The approximately 56 kDa cross-reacting protein is seen only in the ischemic hemisphere (PMCAO). Lanes 1, 2 and 5, 6 are hemispheres dissected out at 6 h after control and ischemia treatments respectively. The symbol – (minus) indicates without PACAP38, that is, 1 μL of 0.9% saline, whereas + (plus) indicates with 1 pmol (1 μL) of PACAP38 injection. Total protein extraction, separation, Western blotting and image analyses procedures are detailed in Methods. Total protein in the Sham (control) and PMCAO (ischemic) hemispheres were stained with Flamingo stain as shown in Additional file 6: Figure S4.