Fig. 5

CCR5 expression in a TBEV-infected patients with CCR5Δ32/wt versus wt/wt genotype. a FACS plots of peripheral blood (PBMC, upper panel) and cerebrospinal fluid (csf, lower panel) obtained on admission to hospital from a TBE patient with CCR5Δ32/wt genotype. Left: T lymphocytes were gated based on CD3 and side scatter dot plot (gate R1). Middle: CD4+CD45RO+ T lymphocytes were gated in selected R1 population based on CD4 and CD45RO dot plot (gate R3). Isotype controls from the same blood and csf samples were used to define the CD45RO+ population. Gate R2 contains CD4+CD45-negative T lymphocytes. Right: a histogram of CD195 expression intensity (dark gray) measured on gate R3 (light gray—isotype control). b CCR5 expression in the activated Th lymphocytes population in the peripheral blood in TBEV-infected patients stratified by CCR5 genotype, measured as in A and expressed as MFI, on admission (I), 10–14 days later (II) and 4–6 weeks later (III). Data from wt/wt homozygotes (n = 20 in I, 25 in II, and 17 in III) and from CCR5Δ32/wt heterozygotes (n = 3 at each time point) compared with healthy controls (C, n = 10 for wt/wt and 5 for CCR5Δ32/wt). Shown are median (horizontal line), quadrilles (box), and minimum and maximum values (whiskers); in TBE CCR5Δ32/wt groups, individual values are shown as circles. *significant difference between genotypes with p < 0.05; the same results were obtained when CCR5-positive cell fraction was analyzed instead of MFI as a measure of CCR5 expression; c CCR5 expression (presented as MFI) in paired csf (CSF) and peripheral blood lymphocyte (PBL) populations from TBEV-infected patients on admission to hospital; values from wt/wt homozygotes are shown as filled diamonds and from two CCR5Δ32/wt heterozygotes as empty symbols; the CCR5 expression in CCR5Δ32/wt patients is low, but there is an apparent enrichment of the csf population in CCR5 expressing cells, analogously to wt/wt homozygotes