Fig. 4

Inflammatory mediators of bilirubin neurotoxicity. a Relative mRNA expression of inflammatory markers. WT and Ugt1 ^-/- mice mRNA cerebellar expression levels of IL18, NFKβ, nNOS, MMP2, MMP9 and TNFα were analysed at the indicated time points by qRT-PCR. For each gene, data were normalized according to the values of the WT samples at P5. For all the experiments, values represent the mean ± SD. Two-way ANOVA test, *p < 0.05. The number of WT and Ugt1 ^-/- was ≥3 in all the experiments and time points. b Representative IF of cerebellar sections from WT and Ugt1 ^-/- mice at P10 using an anti-TNFα antibody (green), co-stained with an anti-GFAP antibody (red) to highlight astrocytes. c Representative IF of cerebellar sections at P10 using an anti-TNFα antibody (green), co-stained with an anti-Iba1 antibody (red) to highlight microglia. d Representative IF of cerebellar sections at P10 using an anti-NFKβ antibody (red), co-stained with an anti-Iba1 antibody (green) to highlight microglia. Scale bar 50 μm. For IF, Hoechst dye (blue) was used to mark nuclei