Fig. 4

IC100 treatment reduces number and activation state of microglia following EAE. a Flow cytometric quantification of CD45^lowCD11b⁺ total microglia and CD45^lowCD11b⁺MHCII⁺ activated microglia in the spinal cord at 35 dpi after EAE. b Representative flow cytometry plots of CD45^lowCD11b⁺ total microglia (green circles) and CD45^lowCD11b⁺MHCII⁺ activated microglia (right panels) from mice treated with vehicle and IC100 (30 mg/kg). Results are expressed as average ± SEM of 5 mice/group, *p < 0.05, Student’s t test; no statistically significant differences were observed by one-way ANOVA with Dunnet’s multiple comparisons test. c Iba1 immunostaining of microglia and infiltrating myeloid cells in the spinal cord of mice treated with vehicle and IC100 (30 mg/kg). White scale bar = 200 μm; yellow scale bar = 50 μm