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Table 1 Antibodies used for flow cytometry analysis of blood, spleen, and brain samples

From: A systemic immune challenge to model hospital-acquired infections independently regulates immune responses after pediatric traumatic brain injury

Antibody Clone Catalog Concentration Fluorophore Company
TruStainFcX(CD16/32) 93 101319 1:100 Biolegend
CD45 30-F11 103108 1:600 FITC Biolegend
CD11b M1/70 564443 1:500 BUV737 BD Biosciences
Ly6C HK1.4 128035 1:200 BV 605 Biolegend
F4/80 CI:A3-1 MCA497A647 1:200 AlexaFluor 647 Bio-Rad
CD192 (CCR2) SA203G11 150605 1:100 BV 421 Biolegend
Ly6G 1A8 127645 1:400 BV 785 Biolegend
7-AAD 420404 1:100 Biolegend
CD3 17A2 100222 1:100 APC-Cy7 Biolegend
CD4 RM4-5 560782 1:800 V500 BD Biosciences
CD8a 53-6.7 100722 1:200 PE-Cy7 Biolegend
CD45R (B220) RA3-6B2 564662 1:200 BUV496 BD Biosciences
Cx3CR1 SA011F11 149006 1:4000 PE Biolegend