Table 1 Antibodies used for flow cytometry analysis of blood, spleen, and brain samples
Antibody | Clone | Catalog | Concentration | Fluorophore | Company |
|---|---|---|---|---|---|
TruStainFcX(CD16/32) | 93 | 101319 | 1:100 | – | Biolegend |
CD45 | 30-F11 | 103108 | 1:600 | FITC | Biolegend |
CD11b | M1/70 | 564443 | 1:500 | BUV737 | BD Biosciences |
Ly6C | HK1.4 | 128035 | 1:200 | BV 605 | Biolegend |
F4/80 | CI:A3-1 | MCA497A647 | 1:200 | AlexaFluor 647 | Bio-Rad |
CD192 (CCR2) | SA203G11 | 150605 | 1:100 | BV 421 | Biolegend |
Ly6G | 1A8 | 127645 | 1:400 | BV 785 | Biolegend |
7-AAD | – | 420404 | 1:100 | – | Biolegend |
CD3 | 17A2 | 100222 | 1:100 | APC-Cy7 | Biolegend |
CD4 | RM4-5 | 560782 | 1:800 | V500 | BD Biosciences |
CD8a | 53-6.7 | 100722 | 1:200 | PE-Cy7 | Biolegend |
CD45R (B220) | RA3-6B2 | 564662 | 1:200 | BUV496 | BD Biosciences |
Cx3CR1 | SA011F11 | 149006 | 1:4000 | PE | Biolegend |