Fig. 4

Analysis of human epileptic brain tissue reveals significant overlap between neuronal hyperexcitability and inflammatory pathologies. a PCA on gene expression profiles from hippocampal tissue obtained from 21 epileptic patients and 22 healthy controls. b CIBERSORTx enrichment score for neutrophils. c Volcano plot depicting differentially expressed genes [log2(fold change) > 1, p-value < 0.05]. d Statistically significant differences in expression of TNF, CCL3, IL1B, CCL2, and PTGS2 between epileptic and control hippocampal tissue. e Statistical significance of the differences in expression of the top ten overrepresented KEGG pathways among differentially regulated genes. f Network diagram portraying connectivity between KEGG gene sets (nodes), where edges represent shared genes. Red edges denote the presence of TNF in the shared gene list between both pathways. g Quantification of occurrences where a given gene appears on a shared gene list (edge). Top ten most frequently occurring genes are shown