Fig. 7

Neurons mount a robust innate immune cytokine response to ZIKV infection. A, B Results of GO enrichment analysis of microarray data derived from primary cortical neurons following ZIKV-MR766 infection (MOI 0.1) compared to PBS-treated controls after 24 h. Selected GO terms focus on cytokine activation or inflammatory transcription factor responses. Tabular results (A) are graphically represented in a bubble plot (B) to demonstrate associations between fold enrichment, FDR, and number of associated genes for each GO term. C–H qRT-PCR analysis was performed measuring cytokine genes Ifna6 (C), Ifnb (D), Ifng (E), Il1b (F), Il6 (G), and Tnfa (H) at indicated timepoints in 8-week-old adult mouse brains following intracranial ZIKV-MR766 infection. n = 5. ns not significant, * p < 0.05, ** p < 0.01, *** p < 0.001. Bars represent group means