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Mitochondrial damage and “plugging” of transport selectively in myelinated, small-diameter axons are major early events in peripheral neuroinflammation
Journal of Neuroinflammation volume 15, Article number: 61 (2018)
Small-diameter, myelinated axons are selectively susceptible to dysfunction in several inflammatory PNS and CNS diseases, resulting in pain and degeneration, but the mechanism is not known.
We used in vivo confocal microscopy to compare the effects of inflammation in experimental autoimmune neuritis (EAN), a model of Guillain-Barré syndrome (GBS), on mitochondrial function and transport in large- and small-diameter axons. We have compared mitochondrial function and transport in vivo in (i) healthy axons, (ii) axons affected by experimental autoimmune neuritis, and (iii) axons in which mitochondria were focally damaged by laser induced photo-toxicity.
Mitochondria affected by inflammation or laser damage became depolarized, fragmented, and immobile. Importantly, the loss of functional mitochondria was accompanied by an increase in the number of mitochondria transported towards, and into, the damaged area, perhaps compensating for loss of ATP and allowing buffering of the likely excessive Ca2+ concentration. In large-diameter axons, healthy mitochondria were found to move into the damaged area bypassing the dysfunctional mitochondria, re-populating the damaged segment of the axon. However, in small-diameter axons, the depolarized mitochondria appeared to “plug” the axon, obstructing, sometimes completely, the incoming (mainly anterograde) transport of mitochondria. Over time (~ 2 h), the transported, functional mitochondria accumulated at the obstruction, and the distal part of the small-diameter axons became depleted of functional mitochondria.
The data show that neuroinflammation, in common with photo-toxic damage, induces depolarization and fragmentation of axonal mitochondria, which remain immobile at the site of damage. The damaged, immobile mitochondria can “plug” myelinated, small-diameter axons so that successful mitochondrial transport is prevented, depleting the distal axon of functioning mitochondria. Our observations may explain the selective vulnerability of small-diameter axons to dysfunction and degeneration in a number of neurodegenerative and neuroinflammatory disorders.
Guillain-Barré syndrome (GBS) is a life-threatening, typically post-infectious, immune-mediated disease of the peripheral nervous system. Severe ascending muscle weakness, caused by dysfunction of peripheral motor fibers, is the best recognized and the most dramatic sign of the disease. Although most patients survive, about half retain a residual neurological deficit, which can be severe . Sensory symptoms have been under-emphasized , but recent studies reveal that these are clinically relevant [3,4,5] and prevalent, with paresthesia being reported by 75%  of patients and pain by 89% . Interestingly, these symptoms often precede muscle weakness. The presence of pain, paresthesia, and abnormal thresholds for thermal and mechanical stimuli  can occur several weeks prior to muscle weakness [8, 9]. Furthermore, following recovery, sensory impairment remains a long-term consequence of GBS, substantially impairing the quality of life [1, 10, 11]. Indeed, a prospective multi-center study conducted 2 years after disease onset identified sensory impairment in over half of the patient population , while another study reported that sensory deficits persist in the legs of 66% of patients between 3 and 6 years after the onset of GBS .
The early presence of sensory signs and symptoms and the long-term deficit suggest that small-caliber myelinated (Aδ) and unmyelinated axons (C fibers) are affected early in the pathogenesis of GBS. Indeed, the temporal pattern of functional impairment of these axons suggests that they are more vulnerable to inflammatory damage than the larger, motor axons. However, the pathophysiological mechanisms underlying the preferential vulnerability of small-diameter axons are unknown.
We have used confocal microscopy to compare the effects of experimental autoimmune neuritis (EAN), a model of GBS, on mitochondrial function and transport in myelinated, large-, and small-diameter axons in vivo at the time of onset of neurological deficits. We found that mitochondria depolarized, fragmented, and stopped trafficking early in the disease in all axons, but in small axons alone, the damaged mitochondria obstructed normal axonal transport of functional mitochondria, causing depletion of mitochondria distal to the site of damage. Mitochondrial blockage of myelinated, small-diameter axons may contribute to other disorders characterized by preferential degeneration of small fibers, including diabetic and chemotherapy-induced neuropathies.
Inflammation at the onset of EAN reduces the number of transported mitochondria in peripheral nerve axons, but not their velocity
To investigate the mechanisms of inflammatory damage to peripheral nerve axons and axonal mitochondria, we immunized mito-S CFP+ mice with peripheral nerve myelin, and Mycobacterium tuberculosis and pertussis toxin as adjuvants. Such immunization results in an autoimmune response against proteins of peripheral nerve myelin in which spinal roots and peripheral nerves are infiltrated by inflammatory cells. EAN manifests first caudally, typically with temporary hind limb and tail weakness or paralysis, ascending rostrally, as occurs in GBS. To study the early pathological events in EAN, we imaged mitochondria in saphenous nerve axons at the first indication of clinical disease expression, typically weight loss and weakness of the tip of the tail. At this time, the vast majority of axons showed normal morphology, indistinguishable from that in time-matched asymptomatic controls and adjuvant controls. However, only in animals with EAN, we observed that the saphenous nerves contained a number of infiltrating cells with the characteristics typical of amoeboid macrophages, including the binding of isolectin B4 (IB4) (green fluorescent label). Also, time-lapse imaging revealed that these cells moved between individual fibers (Additional files 1 and 2: Videos S1 and S2). Interestingly, in some thin axons in the vicinity of the infiltrating cells, the distribution of mitochondria was uneven, with mitochondria in clumps instead of the typically uniform distribution of mitochondria seen in normal axons. Indeed, neither infiltrating cells nor clumps of mitochondria were found in control animals.
We wondered if the clumping of mitochondria may indicate a defect in mitochondrial transport, so we measured this in animals with EAN at disease onset, i.e., mild weakness of tail tip, comparing the findings with transport in immunized but asymptomatic animals (asymptomatic EAN), and in control animals that received adjuvant only (adjuvant controls). Control animals were examined on the same day as their cohort symptomatic animal or the next day. We found no differences between the number of transported mitochondria in adjuvant controls and asymptomatic EAN animals, in either the anterograde (towards the peripheral terminal) or the retrograde (towards the dorsal root ganglion (DRG)) directions. The median number of anterogradely transported mitochondria in adjuvant controls and asymptomatic mice with EAN was 14 (interquartile range (IQR) = 8–22) and 16 (IQR = 9–22), respectively, with the median number of retrogradely transported mitochondria in the two types of control equal to 3 (IQR = 2–7) and 4 (2–6) respectively. However, in animals expressing a neurological deficit, the median number of anterogradely transported mitochondria was reduced by more than half to 6 (2–14.5) in comparison with control groups (Fig. 1a, p< 0.001, non-parametric one-way ANOVA, Dunn’s post-test). The number of retrogradely transported mitochondria [(1 (0–3)) was also significantly lower than in control groups (p < 0.001, non-parametric one-way ANOVA, Dunn’s post-test). Therefore, at the onset of disease expression, the observations reveal infiltrating inflammatory cells within the perineurium and a significant reduction in axonal mitochondrial transport. Interestingly, the overall axonal structure in these fibers appeared normal, and the mitochondrial distribution was unaffected despite the reduction in axonal mitochondrial transport.
As the systemic inflammation alone (adjuvant controls) did not alter mitochondrial transport, we postulated that the infiltrating population of inflammatory cells may be responsible for the observed result. To determine whether the presence of inflammatory cells in the vicinity of axons may cause impairment of mitochondrial transport within those axons, we examined mitochondrial axonal transport in two different experimental paradigms. First, to exclude the possibility that systemic inflammation affects mitochondrial transport, we induced systemic inflammation by an intraperitoneal injection of lipopolysaccharide (LPS). Two to 4 h after LPS injection, when animals exhibit sickness behavior (i.e., reduced activity, piloerection, hyperventilation), we measured mitochondrial transport in saphenous nerve axons. The number of transported mitochondria was similar to that in adjuvant controls and asymptomatic EAN animals, and anterograde and retrograde transport was similar to controls (median anterograde and retrograde transport (±IQR) = 12 (5–17) and 2 (1–6) respectively (Fig. 1a). We then examined whether the presence of inflammatory cells infiltrated amongst the axons impaired mitochondrial transport, as seen at the onset of EAN. We mimicked inflammation in the peripheral nerve by incubating the saphenous nerve axons with activated macrophages. For this purpose, we isolated peritoneal macrophages from naïve mice and incubated them overnight with sciatic nerve excised from another C57BL6 mouse, in the presence of LPS. On the next day, we opened the perineurium of saphenous nerve axons in vivo and exposed them to 500,000 of these activated macrophages which were re-suspended in 200 μl of supernatant. We measured axonal transport 1 h after adding the macrophages to exposed axons. In comparison with adjuvant control and asymptomatic mice, this “applied infiltration” of saphenous nerve by activated macrophages reduced the number of mitochondria moving both anterogradely [median (IQR) = 11 (8–17.5)] and retrogradely [median (IQR) = 2 (1–4)], although this reduction did not reach statistical significance (Fig. 1e) in comparison with either adjuvant control or asymptomatic animals.
We investigated whether the decrease in the number of transported mitochondria was a result of a reduced speed of mitochondria, but we found no differences in transport speed in either direction between the groups (Fig. 1f).
Inflammation during the symptomatic phase of EAN is associated with mitochondrial fragmentation, loss of mitochondrial membrane potential, and focal accumulations of damaged mitochondria
Examination of the length of axonal mitochondria revealed that stationary mitochondria at the onset of EAN were marginally shorter (2.6 ± 1.2 μm) than mitochondria in axons from animals with asymptomatic EAN (2.7 ± 1.3 μm; p < 0.01, one-way ANOVA, Dunns’ post-test), but significantly shorter than mitochondria in adjuvant controls (3 ± 1.5 μm, p < 0.001) (Fig. 2a). This finding indicates that immunization with peripheral nerve antigens may tilt the mitochondrial fusion/fission balance in favor of fission, even when signs of the disease are undetectable or barely detectable. As increased mitochondrial fission has been associated with mitochondrial dysfunction in neurodegenerative diseases [13,14,15], we next examined whether axonal mitochondria maintained their mitochondrial membrane potential (Fig. 2a–b′). Using transgenic mice that constitutively express cyan fluorescent protein in axonal mitochondria independently of mitochondrial membrane potential (i.e., both functional and dysfunctional axonal mitochondria appear blue), we applied the potentiometric dye TMRM (red) to the axons to distinguish functional (positive for CFP with accumulated TMRM, appearing pink or white) from dysfunctional mitochondria (positive for CFP but failing to accumulate TMRM, appearing blue) in vivo. In healthy controls and asymptomatic animals, the vast majority of axons were characterized by mitochondria of various lengths, amongst which both elongated and round shorter mitochondria were clearly labeled with both CFP and TMRM (Fig. 2d, white arrows). However, in animals with EAN, about 10% of axons showed an atypical, focal accumulation of mitochondria (Fig. 2b–e, arrows), and within these accumulations, depolarized mitochondria (blue) were stationary throughout the period of observation (typically over 2 h). Also, the depolarized mitochondria were fragmented, typical of damaged mitochondria . Indeed, given that the fragmented and depolarized mitochondria do not participate in mitochondrial fusion, this effect of inflammation may have contributed to the perceived shortening of axonal mitochondria mentioned above. Interestingly, the focal accumulations, consisting of a mixture of depolarized and polarized mitochondria, appeared gradually to increase in size (Fig. 2b and b′). Furthermore, time-lapse imaging revealed that the accumulations occurred predominantly, although not exclusively, by anterogradely moving, polarized, mitochondria joining the “road block” (Additional files 3 and 4: Videos S3 and S4). Interestingly, only small-diameter myelinated axons seemed to be affected by mitochondrial accumulations (Fig. 2a′). We then measured the mitochondrial density in axons of different calibers and found that, indeed, small-caliber axons tend to host a greater density of mitochondria than large-caliber axons (Fig. 2c, p< 0.0001, r2 = 0.16). The combination of small-diameter and greater mitochondrial density makes small-diameter axons especially vulnerable to plugging when mitochondria are damaged and transport ceases.
Focal mitochondrial damage increases anterograde mitochondrial transport into the damaged area but causes accumulations of mitochondria in small-diameter fibers
We postulated that damaged mitochondria might cause a road block in the path of incoming transported axonal mitochondria. To test this hypothesis, we induced localized mitochondrial damage by photo-toxic bleaching (561 nm) in a well-defined area (Fig. 3, 124 × 124 μm region in A), and measured mitochondrial transport distally and proximally to the bleached region, before and shortly after damage (Fig. 3, regions 1 and 5 in B). We found that distal to the site of photo-toxic mitochondrial damage, the number of anterogradely and retrogradely moving mitochondria did not significantly change (Fig. 3d). However, proximal to the damage, there was a clear increase in anterograde transport in all examined axons (Fig. 3c, p = 0.008, repeated measures ANOVA). Thus, the damaged region of the axons seems repopulated mainly by the increase in number of anterogradely trafficking mitochondria moving into the region. The preponderance of anterogradely moving mitochondria into the photo-bleached region can be observed in time-lapse images of the region obtained within a few minutes after the photo-toxic depletion of mitochondria (Additional file 5: Video S5).
Within the region subjected to photo-bleaching, all the mitochondria initially appeared polarized and uniformly distributed along the axons (Fig. 4a), but then, as expected following photo-bleaching, they all promptly became depolarized (TMRM−, Fig. 4b). The majority of the mitochondria retained their original shape and size, but some mitochondrial fragmentation occurred, including the formation of a small number of short, round mitochondria typical of the damaged form  (Fig. 4, blue arrows in B). Over the following hours, the vast majority of mitochondria within the photo-bleached area underwent fragmentation. These CFP+ TMRM− mitochondrial fragments remained stationary for at least 2.5 h (Fig. 4, blue arrows in C, D, and E). As mentioned above, the photo-bleaching induced an increase in anterograde mitochondrial transport, and these polarized, anterogradely transported mitochondria began to repopulate the damaged region. However, the passage of the healthy, polarized mitochondria towards the distal parts of the axons was hindered by the stationary fragments of mitochondria generated by the photo-bleaching, resulting in the formation of focal mitochondrial accumulations consisting of both polarized and depolarized mitochondria (Fig. 4, white arrows in D). These accumulations steadily increased in size, mainly growing in the proximal direction (Fig. 4, white arrowheads in E), while the distal parts of the axons became depleted of mitochondria. Interestingly, accumulations of mitochondria only occurred in small-diameter, myelinated, fibers measuring 3.02 (± 0.7) μm (mean ± SD, n = 18 axons from three separate experiments). In larger axons, measuring 4.24 (± 1.15) μm (mean ± SD, n = 35 axons from three separate experiments), although some healthy, polarized mitochondria were blocked by damaged fragments, the majority by-passed these “road blocks” to travel onward to the distal axon (annotated by asterisks in Fig. 4). There was a statistically significant difference between the calibers of the axons that were differentially affected by laser photo-toxicity (p < 0.001, Student’s t test).
Clinical and pathological studies implicate dysfunction of small-diameter fibers in the early neurological signs of the most common form of acute peripheral inflammatory polyradiculoneuropathy, Guillain-Barré syndrome (GBS). In this study, we reveal how inflammation within the saphenous nerve is associated with mitochondrial dysfunction in small-diameter fibers, in vivo. The effects involve a sequential process including (i) loss of mitochondrial membrane potential followed by mitochondrial fragmentation, (ii) accumulation of mitochondrial debris at the site of damage, (iii) compensatory increase in anterograde mitochondrial transport proximal to the site of damage, (iv) obstruction of incoming transported mitochondria by the debris, and (v) mitochondrial depletion distal to the site of initial damage. We show that the consequences of this sequence of events are different in small-diameter myelinated axons compared with medium- and large-diameter axons in that greater mitochondrial density and space restrictions render small-caliber axons becoming more susceptible to a complete block of axonal transport of mitochondria at the site of initial mitochondrial damage.
Association between inflammation and mitochondrial damage
At the onset of the signs of EAN, we observed that some axonal mitochondria appeared fragmented and depolarized, and that these were always associated with immobile inflammatory cells that were closely juxtaposed to the axon concerned. It was not clear whether the inflammatory cells were attracted by signals from the damaged axon, or whether the mitochondrial damage was a consequence of the attachment of the inflammatory cell to the axon as a consequence of the anti-nerve immune response, but it seems reasonable to speculate that in this model of active immunization, it was the inflammatory cells that were the instigators of the damage. Indeed, we have not observed mitochondrial damage or disruption of mitochondrial transport in any of the control groups of animals, i.e., animals that received only adjuvants, pyrogenic doses of LPS i.p., or application of in vitro-activated macrophages. Early mitochondrial damage in the context of neuroinflammation has been documented in EAE [17, 18], as well as in MS [19, 20], where soluble inflammatory mediators and reactive oxygen species have been implicated.
Consequences of focal mitochondrial damage for the axons
The likely consequences of loss of functional mitochondria to the affected axon are multiple and complex. One of the most obvious is an energy crisis, with resulting Ca2+ toxicity due to depletion of the ATP required for Ca2+ homeostasis and the release of Ca2+ from damaged mitochondria . It is well established that both a lack of ATP and high Ca2+ concentration are incompatible with long-term axonal survival [22, 23]. Thus, unsurprisingly, an increase in ADP  and Ca2+ have both been implicated in regulating mitochondrial axonal transport towards the area of high energy demand . Such regulated, directional transport of functional mitochondria is vital for axonal survival under different physiological  and pathological conditions [21, 27, 28]. In agreement with these observations, made in ex vivo models, is our finding that focal, photo-toxic damage of axonal mitochondria rapidly induces an increase in anterograde transport of functional mitochondria towards the site of damage, in vivo. The supply of these mitochondria to the affected area would provide the ATP necessary to handle excess Ca2+, preventing axonal dysfunction, and possibly degeneration.
However, another challenge for axons affected by focal mitochondrial damage is the effective disposal of mitochondrial debris. In vitro studies have indicated that damaged axonal mitochondria are transported retrogradely to the cell body in order to be degraded . It was later observed, in cultured hippocampal neurons, that autophagosome formation and subsequent mitophagy may occur without additional transport of damaged mitochondria, i.e., in situ . Indeed, our study shows that in long, adult axons in vivo, damaged mitochondria are not removed from the site of damage and transported elsewhere for at least 3 h following the initial damage. The advantage of the in situ degradation of mitochondria is rapid neuroprotection against oxidative damage without energetically expensive and lengthy retrograde axonal transport . Indeed, the mechanism underlying the detachment of depolarized mitochondria from microtubules, thereby preventing further axonal transport, has been described in rat hippocampal cultures. Namely, depolarized mitochondria are targeted by the PINK1/Parkin pathway, which subsequently initiates proteasome-dependent degradation of the mitochondrial adaptor protein Miro, resulting in release of the motor protein kinesin from mitochondrial surface . Once detached from kinesin, depolarized mitochondria become arrested and likely stabilized in situ by cytoskeletal neurofilament .
An important finding of our study is that the presence of mitochondrial debris, exacerbated by a compensatory increase in axonal transport of functional mitochondria, represents a challenge for axons, especially myelinated axons of small caliber. It should be noted that by small-caliber axons, we do not refer to the classical nociceptors, the unmyelinated (or C) fibers, although a proportion of these axons is strongly, specifically labeled with IB4. Indeed, we have used another characteristic of IB4 to faintly label myelin, in order to visualize and measure the caliber of the small-caliber myelinated axons. By using this method, the caliber of any myelinated axon can be precisely measured, for example using the ZEN imaging software. The increased influx of functional mitochondria mentioned above is likely designed to improve the bio-energetic profile of the affected area of the axon; its spatial co-localization with the stationary mitochondrial debris may be counterproductive. Namely, the accumulated mitochondrial debris in small-diameter axons seems physically to obstruct the passage of the incoming, functional mitochondria from reaching parts of the axon distal to the affected site. In a similar way, a car breakdown on a small road will obstruct passing cars causing a traffic jam, whereas the same car on a wide road will have little consequence. Similarly, in medium- and large-caliber axons, the functional mitochondria can bypass damaged mitochondria. Thus, it appears that the coincidence of damaged mitochondria and a compensatory increase in axonal transport of functional mitochondria represents a challenge axons for small caliber. Indeed, we have shown that mitochondrial density is greater in the small-caliber axons than in the larger ones, which exacerbates the problem.
Further to the accumulation of mitochondrial debris, the damage to mitochondria within the axons will activate mechanisms for removal of such debris, for example via activation of PINK1/Parkin pathway, as mentioned above. The consequence of activation of PINK1/Parkin pathway is the formation of autophagosomes. Our and other recent studies strongly suggest that such formations will form locally, and this will further increase the mass of heterogeneous material likely to obstruct transport in small-caliber axons. It is easy to envisage the formation of a vicious circle aggravating transport deficits following an inflammatory insult and reducing the possibility of recovery. This consideration highlights the opportunities that may arise from enhancing the removal of debris material, such as improved mitophagy and autophagy strategies, in neurodegenerative diseases. Indeed, the distal segments of the affected small-diameter axons had already become depleted of functional mitochondria upon first observation, and this problem got worse during our observation period. Therefore, in vivo, only a few hours may be a sufficient time period for the consequences of blocked axonal transport and metabolic disturbances to become exacerbated. The depletion of mitochondria in the distal axon is likely to contribute to the long-term dysfunction and distal degeneration resulting from inflammatory insult. Indeed, sensory impairment remains a long-term consequence of GBS [10, 11], and it correlates with the density of intra-epidermal nerve fibers (IENF) , suggesting that the dysfunction is progressive, finally resulting in degeneration of the distal axon.
It seems likely that mitochondrial damage and distal depletion of mitochondria precipitated by selective obstruction of small-diameter myelinated axons could play a part in a number of other inflammatory conditions characterized by small fiber degeneration. Indeed, it has been shown in cultured dorsal root ganglia expressing small fiber neuropathy-associated G856D mutant Nav1.7 channels that degeneration of neurites  is promoted by a decrease in ATP levels and increase in intracellular calcium levels [Ca2+]i, which would be expected as a consequence of mitochondrial damage and depletion. Selective loss of small-diameter myelinated axons occurs in CNS in multiple sclerosis , and it is especially prominent in small fiber neuropathies (SFN), which are often characterized by pain and autonomic symptoms, including diabetic neuropathy [37,38,39], chemotherapy-associated neuropathy , HIV , systemic lupus erythematosus , paraneoplastic syndrome , sarcoidosis , drug toxicity [45,46,47], hereditary neuropathies , familial amyloidosis , and inflammatory peripheral neuropathy and Guillain-Barré syndrome (GBS) . The pervasive vulnerability of small-diameter axons is striking, and the mechanisms described in this paper may contribute to their selective vulnerability. If so, research into therapeutic strategies to overcome the formation of a mitochondrial plug, such as stimulating the degradation of mitochondrial debris, may be beneficial target for treating peripheral neuropathies.
The inflammatory insult creates debris of stationary, damaged mitochondria within axons. In small-diameter myelinated axons, the combination of a space restriction and a high density of mitochondrial debris together result in plugging of axons with debris which in turn further obstructs axonal transport and hinders repopulation of the affected segments with healthy mitochondria. The long-term depletion of mitochondria from affected portion and distal axons may contribute to the metabolic impairment, long-term dysfunction, and distal axonal degeneration.
All animal experiments were carried out according to the 1986 Animals (Scientific Procedures) Act, UK, and were approved by the institutional ethics committee. Mice used in this study were male, 8–12 weeks old, and either Thy1-CFP-S-positive or Thy1-CFP-S-negative littermates (Jackson laboratories strain designation: B6;CB-Tg (Thy1-CFP/COX8A)S2Lich/J). In CFP-S mice, the cyan fluorescent protein is constitutively expressed only in axonal mitochondria, affecting approximately 40–60% of them, and thus, the label was used to distinguish axonal from Schwann (and other) cell mitochondria. As the CFP labeled mitochondria fluoresce blue regardless of their membrane potential, we used another probe tetramethylrhodamine methyl ester (TMRM, Invitrogen, UK) which partitions into polarized mitochondria to label polarized (probably healthy) mitochondria selectively.
Induction and assessment of experimental autoimmune neuritis (EAN)
EAN was induced in 8–12-week-old Thy1-CFP-S-positive and Thy1-CFP-S-negative littermates (Jackson laboratories strain designation: B6;CB-Tg(Thy1-CFP/COX8A)S2Lich/J) by two subcutaneous injections, 6 days apart, of an emulsion prepared from saline solutions of peripheral bovine myelin (2.5 mg/mouse), P0106–125 (50 μg/mouse) (Genosphere Biotechnologies, Paris, France) and P251–84 (50 μg/mouse) (a kind gift from Dr. Norman A Gregson) and incomplete Freund’s adjuvant (Sigma, Poole, UK) supplemented with 0.5 mg of heat-inactivated Mycobacterium tuberculosis (Difco, USA). The injections were carried out under isoflurane anesthesia (2% in oxygen). In addition, 200 ng of pertussis toxin (Chemicon, UK) was administered intraperitoneally to each mouse, both at the same time as the first immunization and also 1 day later. At the same time, control mice received the same amount of emulsion prepared from saline and the same doses of adjuvants. Animals were daily weighed and assessed for disease severity using a 10-point scale, one point being given for each of the following signs: reduction of muscle tone of tail tip, reduction of muscle tone of the whole tail, tail paralysis, inability to spread toes, unsteady gait, hind limb paresis (one point per limb), limb paralysis (one point per limb), and moribund. The cumulative severity of motor deficit that each animal exhibited was expressed as the area under the curve of that animal’s motor deficit against time, as calculated using GraphPad Prism software.
Surgical procedure and imaging
On the first day of appearance of neurological signs of EAN (symptomatic EAN group), typically week tip of the tail, mice were anesthetized (no recovery; urethane; 1.25 g/kg) for imaging studies, as previously described . On the same day, or a day after, an adjuvant-only control mouse was examined in the same way. Briefly, the skin was opened under urethane anesthesia at the thigh and the connective tissue carefully removed. Approximately 1 cm of the left saphenous nerve was exposed in the middle of the thigh, and desheathed taking care to avoid any damage to the nerve fibers or the vasculature. The nerve was incubated in situ with tetramethylrhodamine methyl ester (TMRM; 0.5 μM solution in sterile saline; pH = 7.4) for 45 min, followed by Griffonia IB4 (Sigma, UK, 1:20 in sterile saline) for 10 min. Labelling with TMRM in these mice enabled distinguishing between two populations of mitochondria, polarized (healthy) and depolarized (damaged). Thus, as a potentiometric, kationic dye, TMRM accumulates only in mitochondria with preserved membrane potential. Thus, TMRM+ CFP+ mitochondria are considered healthy and in confocal images appear pale pink or white. In contrast, CFP is constitutively expressed in all axonal mitochondria regardless of their membrane potential; thus, TMRM−, CFP+ mitochondria are depolarized and are considered damaged (in confocal images, they appear cyan). Following the labelling, the animal was transferred to the customized stage of a confocal microscope (LSM Pascal 5.0, Zeiss, Germany). Time-lapse or single images of the middle portion of the exposed nerve in the thigh were acquired at 1.97 s per frame using LSM Pascal 5.0 software (Zeiss, Germany) and Zeiss Apochromat Plan × 63 (oil) warmed (36 °C) objective (NA 1.4). The rectal temperature was continuously monitored and maintained at 36 °C by an underlying heating mat. In total, a minimum of three animals from each group were examined.
Focal, photo-toxic damage of axonal mitochondria in vivo
An additional three asymptomatic EAN animals were used to study the effects of photo-toxic damage to axonal mitochondria, in vivo. The saphenous nerve was surgically prepared and labeled with TMRM and IB4, as described above. Three adjacent regions, each measuring 124 μm2 (corresponding the field of view under × 63 objective), were identified along the saphenous nerve (Fig. 3). In order to determine the baseline rate of mitochondrial transport within those regions, time-lapse images of axonal mitochondria were taken as described above. Next, the central of the three regions was photo-bleached by repeated scanning using laser light at 561 nm. The process was repeated until TMRM was entirely photo-bleached. After photo-bleaching, time-lapse images were taken again in the same three regions, i.e., the central, and proximal and distal to central, in order to evaluate mitochondrial function and transport in the same axons as before the photo-toxic damage.
The number, direction, velocity of moving, and the size of axonal mitochondria in the time-lapse videos were analyzed by a blinded examiner using ImageJ. As CFP is present in both healthy and damaged mitochondria, these measurements were performed on the red channel (i.e., the TMRM labelling) to exclude damaged mitochondria from skewing the analyses of mitochondrial transport. However, the CFP labelling was used to confirm that the analyzed particles were indeed axonal, and not Schwann cell, mitochondria. Also, mitochondrial length in axons was determined in both channels, separately. Internodal segments of myelinated axons with caliber between 4 and 7 μm were first straightened using the Straighten plug-in (http://rsbweb.nih.gov/ij/plugins/straighten.html) for ImageJ version 1.43 software (NIH, Bethesda, MD, USA, rsb.info.nih.gov/ij/). Straightened axons were processed using the Difference Tracker plug-in (https://www.bioinformatics.babraham.ac.uk/projects/difference_tracker/) for ImageJ as previously described [50, 51], and fluorescent particles with a lower cutoff of 5 pixels moving for at least six consecutive frames were considered to be moving mitochondria. The lower cutoff was determined by examination of the size of the smallest moving CFP and TMRM+ particles. The number of moving mitochondria and their direction and velocity were directly derived from the results table generated by the Mass Particle Tracker component of the Difference Tracker plug-in. Length of stationary mitochondria was analyzed using the ImageJ Analyse Particle plug-in. First, time-lapse images of axons were straightened using the Straighten plug-in, the background was reduced using intensity subtraction (the radius of the rolling ball was set to 5) in ImageJ, and the time lapse was converted into a binary form. Using the drawing tool, all the moving particles were eliminated. The dimensions of the remaining stationary particles were analyzed using the Analyse Particle plug-in for ImageJ, whereby the Feret’s diameter value was converted into length in micrometers.
Prior to all statistical analyses, the data were tested for normality of distribution using the D’Agostino and Pearson normality test. To calculate differences between the number of track counts and the velocity of mitochondrial transport in different groups of animals, non-parametric one-way ANOVA with Dunn’s multiple comparison post-test was used. However, to calculate the difference between the number of mobile mitochondria before and after laser photo-toxic damage, we used repeated measures ANOVA. Differences in length of mitochondria are tense change calculated using one-way ANOVA with Dunn’s multiple comparison tests. Differences between the numbers of small-diameter axons with detected accumulations in different groups were calculated using Student’s t test. Parametric data are presented as mean ± SEM, whereas non-parametric, or data which did not follow a normal distribution, are presented as median ± interquartile range (IQR).
Analysis of variance
Cyan fluorescent protein
Central nervous system
Dorsal root ganglion
Experimental autoimmune encephalomyelitis
Experimental autoimmune neuritis
Human immunodeficiency virus
Intra-epidermal nerve fibers
Laser scanning microscope
PTEN-induced putative kinase 1
Peripheral nervous system
Standard error of the mean
Small fiber neuropathy
Tetramethylrhodamine methyl ester
de la Dornonville Cour C, Jakobsen J. Residual neuropathy in long-term population-based follow-up of Guillain-Barré syndrome. Neurology. 2005;64:246–53.
Karkare K, Taly AB, Sinha S, Rao S. Temporal profile of pain and other sensory manifestations in Guillain-Barré syndrome during ten days of hospitalization. Neurol India. 2011;59(5):712–6. https://doi.org/10.4103/0028-3886.86546.
Wakerley BR, Yuki N. Isolated facial diplegia in Guillain-Barré syndrome: bifacial weakness with paresthesias. Muscle Nerve. 2015;52:927–32.
Hayashi R, Yamaguchi S. Guillain-Barré syndrome variant with facial diplegia and paresthesias associated with IgM anti-GalNAc-GD1a antibodies. Intern Med. 2015;54:345–7.
Biotti D, et al. Blindness, weakness, and tingling. Surv Ophthalmol. 2012;57:565–72.
Moulin DE, Hagen N, Feasby TE, Amireh R, Hahn A. Pain in Guillain-Barré syndrome [see comments]. Neurology. 1997;48:328–31.
Martinez V, et al. Small fibre impairment predicts neuropathic pain in Guillain-Barré syndrome. Pain. 2010;151:53–60.
Ruts L, et al. Pain in Guillain-Barré syndrome: a long-term follow-up study. Neurology. 2010;75:1439–47.
Boukhris S, Magy L, Khalil M, Sindou P, Vallat JM. Pain as the presenting symptom of chronic inflammatory demyelinating polyradiculoneuropathy (CIDP). J Neurol Sci. 2007;254:33–8.
Rekand T, Gramstad A, Vedeler CA. Fatigue, pain and muscle weakness are frequent after Guillain-Barré syndrome and poliomyelitis. J Neurol. 2009;256:349–54.
Forsberg A, et al. Impairment in Guillain–Barré syndrome during the first 2 years after onset: a prospective study. J Neurol Sci. 2004;227:131–8.
Bernsen RA, Jager AE, Schmitz PI, van der Meché FG. Long-term sensory deficit after Guillain-Barré syndrome. J Neurol. 2001;248:483–6.
Barsoum MJ, et al. Nitric oxide-induced mitochondrial fission is regulated by dynamin-related GTPases in neurons. EMBO J. 2006;25:3900–11.
Cho D-H, et al. S-Nitrosylation of Drp1 mediates beta-amyloid-related mitochondrial fission and neuronal injury. Science. 2009;324:102–5.
Bossy-Wetzel E, Barsoum MJ, Godzik A, Schwarzenbacher R, Lipton SA. Mitochondrial fission in apoptosis, neurodegeneration and aging. Curr Opin Cell Biol. 2003;15:706–16.
Kaasik A, Safiulina D, Zharkovsky A, Veksler V. Regulation of mitochondrial matrix volume. AJP Cell Physiol. 2006;292:C157–63.
Sadeghian M, et al. Mitochondrial dysfunction is an important cause of neurological deficits in an inflammatory model of multiple sclerosis. Sci Rep. 2016;6(1):33249.
Nikić I, et al. A reversible form of axon damage in experimental autoimmune encephalomyelitis and multiple sclerosis. Nat Med. 2011;17:495–9.
Lu F, et al. Oxidative damage to mitochondrial DNA and activity of mitochondrial enzymes in chronic active lesions of multiple sclerosis. J Neurol Sci. 2000;177:95–103.
Mahad DJ, et al. Mitochondrial changes within axons in multiple sclerosis. Brain. 2009;132:1161–74.
Celsi F, et al. Mitochondria, calcium and cell death: a deadly triad in neurodegeneration. Biochim Biophys Acta Bioenerg. 2009;1787:335–44.
Abeti R, Abramov AY. Mitochondrial Ca2+ in neurodegenerative disorders. Pharmacol Res. 2015;99:377–81.
Arun S, Liu L, Donmez G. Mitochondrial biology and neurological diseases. Curr Neuropharmacol. 2016;14:143–54.
Mironov SL. ADP regulates movements of mitochondria in neurons. Biophys J. 2007;92:2944–52.
Saotome M, et al. Bidirectional Ca2+-dependent control of mitochondrial dynamics by the Miro GTPase. Proc Natl Acad Sci U S A. 2008;105:20728–33.
Sajic M, et al. Impulse conduction increases mitochondrial transport in adult mammalian peripheral nerves in vivo. PLoS Biol. 2013;11:e1001754.
Saxton WM, Hollenbeck PJ. The axonal transport of mitochondria. JCell Sci. 2012;125:2095–104.
López-Doménech G, et al. Loss of dendritic complexity precedes neurodegeneration in a mouse model with disrupted mitochondrial distribution in mature dendrites. Cell Rep. 2016;17:317–27.
Maday S, Wallace KE, Holzbaur ELF. Autophagosomes initiate distally and mature during transport toward the cell soma in primary neurons. J Cell Biol. 2012;196:407–17.
Ashrafi G, Schlehe JS, LaVoie MJ, Schwarz TL. Mitophagy of damaged mitochondria occurs locally in distal neuronal axons and requires PINK1 and Parkin. J Cell Biol. 2014;206:655–70.
Schwarz TL. Mitochondrial trafficking in neurons. Cold Spring Harb Perspect Biol. 2013;5:a011304.
Wang X, et al. PINK1 and Parkin target Miro for phosphorylation and degradation to arrest mitochondrial motility. Cell. 2011;147:893–906.
Wagner OI, et al. Mechanisms of mitochondria-neurofilament interactions. J Neurosci. 2003;23:9046–58.
Ruts L, et al. Unmyelinated and myelinated skin nerve damage in Guillain-Barré syndrome: correlation with pain and recovery. Pain. 2012;153:399–409.
Rolyan H, et al. A painful neuropathy-associated Nav1.7 mutant leads to time-dependent degeneration of small-diameter axons associated with intracellular Ca2+ dysregulation and decrease in ATP levels. Mol Pain. 2016;12:1–13.
DeLuca GC, Ebers GC, Esiri MM. Axonal loss in multiple sclerosis: a pathological survey of the corticospinal and sensory tracts. Brain. 2004;127:1009–18.
Sumner CJ, Sheth S, Griffin JW, Cornblath DR, Polydefkis M. The spectrum of neuropathy in diabetes and impaired glucose tolerance. Neurology. 2003;60:108–11.
Tesfaye S, et al. Diabetic neuropathies: update on definitions, diagnostic criteria, estimation of severity, and treatments. Diabetes Care. 2010;33:2285–93.
Smith AG, Ramachandran P, Tripp S, Singleton JR. Epidermal nerve innervation in impaired glucose tolerance and diabetes-associated neuropathy. Neurology. 2001;57:1701–4.
Kannarkat G, Lasher EE, Schiff D. Neurologic complications of chemotherapy agents. Curr Opin Neurol. 2007;20:719–25.
Polydefkis M, et al. Reduced intraepidermal nerve fiber density in HIV-associated sensory neuropathy. Neurology. 2002;58:115–9.
Tseng M-T, et al. Skin denervation and cutaneous vasculitis in systemic lupus erythematosus. Brain. 2006;129:977–85.
Oki Y, et al. Ataxic vs painful form of paraneoplastic neuropathy. Neurology. 2007;69:564–72.
Hoitsma E, et al. Small fibre neuropathy in sarcoidosis. Lancet. 2002;359:2085–6.
Giannoccaro MP, et al. Somatic and autonomic small fiber neuropathy induced by bortezomib therapy: an immunofluorescence study. Neurol Sci. 2011;32:361–3.
Heckmann JG, Dütsch M, Schwab S. Linezolid-associated small-fiber neuropathy. J Peripher Nerv Syst. 2008;13:157–8.
Burakgazi AZ, Polydefkis M, Höke A. Skin biopsy-proven flecainide-induced neuropathy. Muscle Nerve. 2012;45:144–6.
Traugott U, Reinherz EL, Raine CS. Multiple sclerosis. Distribution of T cells, T cell subsets and Ia-positive macrophages in lesions of different ages. J Neuroimmunol. 1983;4:201–21.
Planté-Bordeneuve V, Said G. Familial amyloid polyneuropathy. Lancet Neurol. 2011;10:1086–97.
Andrews S, Gilley J, Coleman MP. Difference tracker: ImageJ plugins for fully automated analysis of multiple axonal transport parameters. J Neurosci Methods. 2010;193:281–7.
Gilley J, et al. Age-dependent axonal transport and locomotor changes and tau hypophosphorylation in a ‘P301L’ tau knockin mouse. Neurobiol Aging. 2012;33(3):621. e1–621.e15.
We thank Vincenzo Mastrolia and Mona Sadeghian for the expert technical advice and assistance.
This work was supported by grants from Medical Research Council (UK) and Multiple Sclerosis Society of Great Britain and Northern Ireland.
Availability of data and materials
Please contact the author for data requests.
All animal experiments were carried out according to the 1986 Animals (Scientific Procedures) Act, UK, and were approved by the institutional ethics committee.
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The authors declare that they have no competing interests.
Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.
Video S1. High-power time-lapse video of inflamed saphenous nerve on the first day of onset of EAN taken immediately upon exposing the saphenous nerve. Axonal myelin (clearly visible around node of Ranvier) is labeled with IB4+ (green) and functional mitochondria are labeled with TMRM (red). Some of the axonal mitochondria are mobile. In between axons, a number of infiltrating inflammatory cells labeled with IB4+ (surface label, green arrows) can be seen, including the mitochondria (TMRM, red) within the cells. Most inflammatory cells appear freely mobile and moving in typical amoeboid fashion between the axons. In one axon (white arrowhead), functional mitochondria appear focally accumulated, as opposed to uniformly distributed mitochondrial in surrounding axons. One of the inflammatory cells, encircled, can be seen juxtaposed to this focal accumulation. (AVI 3211 kb)
Video S2. High-power time-lapse video of inflamed saphenous nerve on the first day of onset of EAN, taken 40 min after the exposure of saphenous nerve. Forty minutes following the first time-lapse video (Additional file 1: Video S1), the same axons are apparent in the field of view. Some mobile inflammatory cells (surface labeled with IB4+, green) and functional mitochondria (TMRM, red) can be seen. Notably, one inflammatory cell (encircled) can be seen closely juxtaposed to the same axon as seen 40 min earlier, still stationary. This is likely to be the same cell, remaining in the close proximity to the axon throughout the 40-min period. The accumulation of axonal mitochondria within this axon appear extended, with some loss of bright red TMRM labelling (mitochondrial membrane potential), suggesting possible mitochondrial damage in that area. (AVI 2879 kb)
Video S3. High-power time-lapse video of axonal mitochondria labeled with TMRM on the day of onset of EAN taken immediately upon exposing the saphenous nerve. Focal accumulation of functional axonal mitochondria (yellow arrow) is observed, with mitochondria moving in anterograde direction. Distal to the accumulation, axons appear depleted of functional mitochondria. In contrast, mitochondrial distribution and morphology appears normal in another axon (green arrow) which is of larger caliber than the two axons affected by mitochondrial accumulation. The video is shown in gray scale in order to improve contrast. (AVI 3253 kb)
Video S4. High-power time-lapse video of axonal mitochondria labeled with TMRM on the day of onset of EAN taken 80 min upon exposing the saphenous nerve. Eighty minutes following the Additional file 3: Video S3, the focal accumulations of functional axonal mitochondria (yellow arrow) appear enlarged in comparison with the earlier time point. Anterograde mitochondrial movement in these axons seems unaffected. Notably, in an axon positioned between the two axons with accumulations (green arrow) and which is of larger diameter than the two indicated with yellow arrows, mitochondrial distribution and morphology appear normal. The video is shown in gray scale in order to improve contrast. (AVI 1353 kb)
Video S5. High-power time-lapse video of axonal mitochondria labeled with TMRM immediately following laser-induced phototoxic damage to mitochondria. The side of the imaging field left to the white line was not exposed to photo-toxic damage. The imaging field to the right of the white line was exposed to laser-induced photo-toxic damage, using the red laser to specifically damage functional mitochondria. Notably, the number of mobile mitochondria transported into the area exposed to photo-toxic damage appears overwhelmingly biased in the favor of anterograde movement. (AVI 2466 kb)