Time-dependent effects of CX3CR1 in a mouse model of mild traumatic brain injury
© Febinger et al. 2015
Received: 23 March 2015
Accepted: 20 August 2015
Published: 2 September 2015
Neuroinflammation is an important secondary mechanism that is a key mediator of the long-term consequences of neuronal injury that occur in traumatic brain injury (TBI). Microglia are highly plastic cells with dual roles in neuronal injury and recovery. Recent studies suggest that the chemokine fractalkine (CX3CL1, FKN) mediates neural/microglial interactions via its sole receptor CX3CR1. CX3CL1/CX3CR1 signaling modulates microglia activation, and depending upon the type and time of injury, either protects or exacerbates neurological diseases.
In this study, mice deficient in CX3CR1 were subjected to mild controlled cortical impact injury (CCI), a model of TBI. We evaluated the effects of genetic deletion of CX3CR1 on histopathology, cell death/survival, microglia activation, and cognitive function for 30 days post-injury.
During the acute post-injury period (24 h–15 days), motor deficits, cell death, and neuronal cell loss were more profound in injured wild-type than in CX3CR1−/− mice. In contrast, during the chronic period of 30 days post-TBI, injured CX3CR1−/− mice exhibited greater cognitive dysfunction and increased neuronal death than wild-type mice. The protective and deleterious effects of CX3CR1 were associated with changes in microglia phenotypes; during the acute phase CX3CR1−/− mice showed a predominant anti-inflammatory M2 microglial response, with increased expression of Ym1, CD206, and TGFβ. In contrast, increased M1 phenotypic microglia markers, Marco, and CD68 were predominant at 30 days post-TBI.
Collectively, these novel data demonstrate a time-dependent role for CX3CL1/CX3CR1 signaling after TBI and suggest that the acute and chronic responses to mild TBI are modulated in part by distinct microglia phenotypes.
KeywordsCX3CR1 TBI Microglia Cognitive function Cytokines
Traumatic brain injury (TBI) is a serious public health problem in the United States. Mild TBI is rarely recognized in a timely manner because symptoms often do not manifest for some time after the injury. However, the consequences of mild TBI can be long lasting. The pathophysiology of TBI is complex, in part because multiple pathways are involved in the primary and secondary injuries that result from the insult.
A consensus based on recent evidence considers neuroinflammation as an important secondary mechanism that plays a role in delaying injury after TBI [1–3]. Dysregulated and uncontrolled microglial activation may be a key component of chronic neuroinflammatory processes. Recent studies suggest that the chemokine fractalkine (CX3CL1, FKN) mediates neural/microglial interactions via its sole receptor CX3CR1. Indeed, deleting CX3CR1  exacerbates microglial neurotoxicity induced by systemic inflammation in rodent models of Parkinson’s disease and amyotrophic lateral sclerosis . In addition, CX3CR1−/− microglia exacerbate cell-autonomous microglial neurotoxicity induced by lipopolysaccharide (LPS), suggesting that fractalkine signaling is important for limiting microglia toxicity [5, 6]. CX3CL1 also protects against excitotoxicity through the activation of the ERK1/2 and PI3K/Akt pathways [7, 8]. However, the role of FKN/CX3CR1 signaling in neurodegenerative disease is an intricate and highly debated research topic that is becoming even more complicated as new studies reveal seemingly discordant results. It appears that FKN/CX3CR1 signaling plays a direct role in neurodegeneration and/or neuroprotection depending upon the CNS insult. Both beneficial and detrimental effects of CX3CR1 deficiency are associated with microglia activation [9–11]. Data from some models of chronic neuronal injury suggest that CX3CR1 is protective because the absence of this receptor increases microglia activity and is associated with less favorable outcome [8, 12–16]. For instance, in a model of Alzheimer’s disease (AD), CX3CR1-deficient microglia from mice overexpressing hTau have increased hyperphosphorylated tau and more toxicity, as has been observed in other animal’s disease models [5, 17–20]. On the other hand, in 3x-Tg AD mice, which reproduces both Aβ and tau pathologies, deleting CX3CR1 prevents neuronal loss and microglial migration without affecting amyloid deposition . Finally, other reports indicate that CX3CR1 deficiency attenuates amyloid deposition in AD mouse models characterized by extensive plaque deposition [18, 22].
The complex CX3CL1/CX3CR1 signaling pathway is further complicated by its functional role in acute injuries. Results from some acute injury models suggest that CX3CR1 is detrimental because its absence is associated with a favorable outcome. For instance, in a model of cerebral ischemia and spinal cord injury, CX3CR1−/− mice showed neuroprotection and improved functional recovery [23–26]. These observations suggest that CX3CR1 may play different roles in neuronal injury depending on the type of injury and on the post-injury time point assessed (chronic vs acute). CX3CL1 levels increase in the cerebrospinal fluid and in the brain of patients with head trauma, and increased CX3CL1/CX3CR1 is associated with better clinical outcome [27, 28]. However, few pre-clinical studies of TBI focus on the CX3CL1/CX3CR1 system. Determining a role for CX3CL1/CX3CR1 signaling in outcomes after mild TBI during the acute and chronic phase is a goal of the present study.
We now know that the microglia assume diverse phenotypes in response to specific microenvironmental signals. Although there is a spectrum of different microglial types, there are two major microglia phenotypes characterized by a molecular signature of gene expression: the “classical” inflammatory state (M1) and the “alternative” activation state (M2). M1 microglia are generally considered pro-inflammatory whereas, M2 microglia are generally anti-inflammatory [29–34]. In the healthy young brain, M1 and M2 microglia exist in a state of dynamic equilibrium. Following an injury, there is a shift into an M1 phenotype that exacerbates tissue injury or into an M2 phenotype that promotes CNS repair, depending on the local signals in the lesion’s microenvironment .
In this study, we demonstrate that lack of CX3CL1/CX3CR1 signaling in the brain leads to delayed neuronal damage and neurologic and cognitive impairment after TBI that is associated with a switch in microglia phenotype. Our data suggest a time-dependent effect of CX3CR1 on modulating brain injury and suggests a potential unique therapeutic window for treating TBI neuroinflammation.
Materials and methods
All experiments were conducted in accordance with the National Institute of Health Guide on Care and Use of Laboratory Animals and were approved by the Institutional Animal Care and Use Committee of the University of Washington. CX3CR1−/− (CX3CR1 GFP/GFP ) mice were obtained from The Jackson Laboratory (Bar Harbor, Maine) and a colony established at the University of Washington. A total of 180 3-month-old male CX3CR1−/− and littermate CX3CR1+/+ (wild-type) mice were used in these experiments. Mice were group-housed in environmentally controlled conditions (12:12 h light-to-dark cycle at 21 ± 1 °C) and provided with food and water ad libitum. After surgery, animals were single-housed.
Traumatic brain injury
Adult mice were anesthetized with isoflurane (induced at 4 %, maintained at 1.25–1.5 %) and placed in a stereotaxic frame equipped with a heating pad to maintain body temperature. A midline incision was made, fascia removed, and a 5-mm diameter craniotomy was made over the left parietal cortex between the lambda and bregma. The bone flap was removed and the integrity of the underlying dura assessed. TBI was induced using a controlled cortical impact (CCI) device (Leica Impact One, Richmond, IL, USA) equipped with an electrically driven 3-mm diameter metal piston controlled by a linear velocity displacement transducer. Impact velocity was set at 5.0 m/s, with a dwell time of 100 ms, and a depth of 0.5 mm from the dura. Previous studies have reported that these parameters produce a mild injury [36, 37]. A 5-mm disk created from a polystyrene weighing boat was glued to the skull over the craniotomy, and the incision closed with sutures. Mice were kept on a heating pad and allowed to recover until ambulatory before being returned to their home cages. Sham (uninjured control) animals received identical anesthesia and craniotomy but were not subjected to CCI brain injury.
Tissue collection and processing
For immunohistochemistry studies, animals were anesthetized with isofluorane and transcardially perfused with ice-cold phosphate-buffered saline (PBS), followed by 4 % paraformaldehyde in PBS. The brains were postfixed in 4 % paraformaldehyde for 24 h, after which they were transferred to a 30 % sucrose in PBS for at least 16 h at 4 °C. Coronal sections of the left hemisphere were made at 40 μm using a cryostat (Leica CM1950) and stored in cryoprotectant at 4 °C. Animals used for protein assessment were euthanatized by rapid decapitation. Brain regions of interest were dissected and rapidly frozen in liquid nitrogen before storage at −80 °C. Subsequently, both the hippocampi and the sections of cortex were homogenized using an electric tissue homogenizer in 1:10 weight-to-volume ratio of ice-cold RIPA buffer (Millipore; Billerica, MA, USA) containing protease inhibitors and EDTA (Pierce; Rockford, IL, USA). Following homogenization, sample lysates were centrifuged at 10,000×g at 4 °C for 15 min and the supernatant collected.
Enzyme-linked immunosorbent assay (ELISA)
The total protein in each sample was determined using the bicinchoninc acid method (BCA; Pierce Biotechnology, Inc.).
Analysis of gene expression by qRT-PCR
Dissected cortex tissues from adult male mice were stored at −80 °C, and total RNA was isolated with RNeasy Lipid Tissue Mini Kit (QIAGEN, catalog # 74804) with on-column DNase treatment (QIAGEN, catalog # 79254) according to the manufacturer’s protocol. RNA quantity and quality were determined using A260/A280 readings by a NanoDrop (ThermoScientific) spectrophotometer. Reverse transcription (RT) was performed using High Capacity cDNA Reverse Transcription Kit Assay (Applied Biosystems, catalog # 4368814) following the manufacturer’s protocol. “No template” and “no RT” controls were included in all assays.
RT-PCR was performed using the TaqMan Gene Expression Assay (Applied Biosystems, catalog # 4331182) according to the manufacturer’s instructions on a 7300 Real Time PCR System (Applied Biosystems). The following TaqMan probes were used: Il-1β (Mm00434228_m1), Cd86 (Mm0444543_m1), Fcgr1 (Mm0438874_m1), Tgfb1 (Mm01178820_m1), Arg1 (Mm00475988_m1), Nos2 (iNOS), (Mm00440502_m1), and 18S (Mm03928990_g1). Relative gene expression was calculated by 2−ΔΔCT method.
Except where indicated, staining was done on 40-μm free-floating sections of a one-in-six series through the entire hippocampus and cortex. Tissue was blocked in 10 % normal serum from the species in which the secondary antibody was raised, with the addition of 0.1 % Triton X-100. Primary antibodies were diluted in 3 % normal serum with 0.1 % Triton X-100, and tissue incubated overnight at 4 °C. Biotinylated secondary antibodies were diluted in 3 % normal serum with 0.1 % Triton X-100 and were incubated for 2 h at room temperature. Enzyme detection was done using avidin-biotin substrate (ABC kit, Vector Laboratories, Burlingame, CA, USA) followed by color development in diaminobenzidine solution (Sigma-Aldrich, St. Louis, MO, USA). Antibodies and dilutions were as follows: rat CD11b (Serotec, Raleigh, NC, USA; 1:500); mouse CD68 (Serotec; 1:200); rabbit YM1 (Stem cell technology, Vancouver, BC, Canada; 1:400); rat Marco (Serotec; 1:500); mouse NeuN (Millipore, Temecula, CA, USA; 1:1000). After incubation for 24–48 h with appropriate primary and biotinylated secondary antibodies, tissue was treated with Vectastain ABC reagent (Vector Labs) and visualized with DAB reaction (Sigma-Aldrich). Controls included omitting primary or secondary antibodies.
Fluoro-Jade B staining
Fluoro-Jade B staining was performed according to Kubova et al. . Briefly, 40-μm coronal free-floating sections were washed three times for 10 min with 0.1 M phosphate buffer saline (PBS), mounted on glass slides, and dried overnight at 37 °C. The sections were then incubated in chloroform-ethanol (1:1) for 1 h. Next, tissue was rehydrated in absolute ethanol (3 min), 70 % ethanol (2 min), distilled water (2 min), incubated in 0.06 % KMnO4 for 15 min, rinsed in distilled water (2 min), and incubated for 30 min in a solution containing 0.001 % Fluoro-Jade B (Histo-Chem, Jefferson, AR, USA) and 0.1 % acetic acid. Sections were then rinsed in distilled water, dehydrated in a series of xylene and coverslipped.
All cell counts were obtained using the Optical Fractionator method of unbiased stereological counting techniques . An Olympus BX-51 microscope and the Stereo Investigator software (MBF Bioscience, Colchester, VT, USA) were used to obtain cell counts on sections systematically sampled throughout the entire hippocampus and parietal cortex as described earlier . Neuroanatomical borders of the hilus of the dentate gyrus were outlined, and counting was confined to these areas. The virtual grid (175 × 175) and counting frame (100 × 100) were optimized to count at least 200 cells per animal with error coefficients less than 0.07. Outlines of the anatomical structures were made using a 10×/0.45 objective, and cell quantification was done with a 60×/1.40 objective.
Isolation of mononuclear cells and flow cytometry
Perfused brains were dissected from mice 15 and 30 days post-TBI, mononuclear cells separated over discontinuous 70/30 % percoll gradients as previously described [41, 42], and cellular pellets resuspended in cell staining buffer (Biolegend, San Diego, CA, USA). Blood for single stained controls was collected from the submandibular vein and RBCs depleted by hypotonic lysis and washed in staining buffer. Isolated cells were incubated on ice for 5 min with anti-mouse CD16/CD32 (clone 2.4G2; BD Pharmingen) to block Fc fragment receptor (FcR)s and then incubated on ice for 30 min with a mix of fluorochrome-conjugated anti-mouse Abs; CD45-APC-Cy7 or APC (Clone 30-F11, BD Pharmingen), CD11b-APC or PerCP-Cy5.5 (Clone M1/70), CD11c-PeCy7 (Clone N418, eBioscience), Ly6G-PE (Clone 1A8, Biolegend), and I-A/I-E-PerCP or Pacific Blue (Mouse MHC-II Clone M5/114.15.2). After washes, cells were resuspended in 2 % paraformaldehyde and analyzed in a LSR-II (BD Biosciences, Franklin Lakes, NJ, USA). To quantify the proportion of resident and infiltrating myeloid cells undergoing cell proliferation, CNS mononuclear cells were stained first with cell surface markers, fixed with 4 % PFA for 30 min and then incubated at RT for 10 min in permeabilization buffer (eBioscience). Cells were then stained with Ki67-V450 (Clone B56) in permeabilization buffer for 20 min, washed, resuspended in 2 % PFA, and acquired on an LSR-II .
Evaluation of neuromotor impairment following CCI was performed by using a 28-point composite neuroscore. In brief, mice were evaluated by an investigator blinded to the injury status (sham, TBI) of the animal. Scoring for each animal ranged from 0 (severely impaired) to 4 (normal strength or function) for each of the following modalities: (1) left and (2) right forelimb flexion during suspension by the tail; (3) left and (4) right hind limb flexion with the forelimbs remaining on a flat surface as the hind limbs are lifted by the tail; (5) ability to resist lateral pulsation to the left and (6) right; and (7) ability to stand on an inclined plane in the left, (8) right, and (9) vertical position. In addition, the ability of mice to stand on an inclined plane for at least 5 s in each of three directions (facing up, left, and right) was determined. The angle was increased in 2.5° increments starting at 40°; the maximal angle was noted and compared with pre-injury (baseline) values. A score was then assigned based on the decrease from baseline angle in degrees, where 4 = 0° change, 3 = 2.5° change, 2 = 5° change, 1 = 7.5° change, and 0 = 10° change or greater. Each of the three directions was scored separately, after which a mean was calculated, giving a maximum score of 4 for the inclined plane. The scores for (7), (8), and (9) were averaged, and a composite neurological motor score (0–28) was calculated by summation of individual test scores. Neuromotor function was assessed in both injured and sham-operated animals, and baseline composite neuromotor scores were calculated from values obtained 24 h prior to injury.
Open-field and elevated plus maze
The open-field and elevated plus maze were used to determine general activity levels and to measure anxiety-like behavior. Animals were monitored under moderate lighting for 15 min in a 40-cm2 open field using video tracking software (ANY-Maze, Stoelting, IL, USA). General activity was evaluated by determining the total of distance traveled. Anxiety-like behavior was assessed based on the pattern of exploration in the open field (center versus periphery). Anxiety-like behavior was also assessed using the elevated plus maze. Our elevated plus maze consists of two well-lit open arms (35 cm) facing each other and two enclosed arms (30.5 cm) also facing each other. Each arm is attached to a common center platform (4.5 cm2), and the entire apparatus was elevated 40 cm off the floor. The mice were placed in the center platform and allowed to explore for 5 min. Video tracking software (ANY-Maze) measured movement and time spent in each section.
Mice were tested for overall balance, motor coordination, and motor learning on an accelerating rota-rod apparatus (Ugo Basile, Italy). The center rotor is a 3-cm diameter cylinder, and rotation started at 4 rpm and accelerated to 40 rpm within a 5-min period. Mice were tested for the time spent on the rotor during each of four trials with a 30-min inter-trial interval, and time to fall off the rotor was the outcome measured.
Morris water maze
The Morris hidden platform water maze (MWM) consisted of a circular pool (1.38 m diameter) filled with room temperature water containing non-toxic opaque paint with an escape platform (10 cm diameter) hidden 3 cm beneath the surface. Each mouse was placed in the pool in a pseudorandom order and given 60 s to locate the escape platform. When the mouse found the platform, or if it failed to find the platform within 60 s, it was placed on the platform and left for 30 s. Each mouse was given four trials per day for 7 days with 1-h inter-trial interval between trials. Sessions were recorded, and the time to find the platform (escape latency), the total distance traveled, and swim speed were determined by video tracking software (ANY-Maze). After each session, mice were towel-dried and placed in a cage on a heating pad until dry, after which they were returned to their home cage. On day 8 post-training, all mice were subjected to one probe trial in which the platform was removed and each animal had 60 s to search the pool for the platform.
Statistical analyses comparing two genotypes, two manipulations (sham and TBI), and two time points (15 and 30 days post-injury) were done using a one-way analysis of variance (ANOVA) or two-way ANOVA with post hoc multiple comparison analysis (Tukey-Kramer or Bonferroni post hoc test). Repeated measures ANOVA followed by Bonferroni post hoc comparisons were used to analyze rota-rod data. Flow cytometry data are presented as number of cells or percentage of specific cell populations. Differences between groups were analyzed using ANOVA or an unpaired t test with GraphPad Prism software (San Diego, CA, USA). P values are shown in the data (*) when P < 0.05. Statistical analysis was done in the GraphPad Prism software.
Mild TBI impairs neuromotor function
Total microglia cell numbers in the brain tissue. Microglia cell numbers in sham WT, sham KO, WT-TBI, and KO-TBI at 15 and 30 days post-TBI as plotted in Fig. 4b. Total microglia cell numbers in the brain tissue. Microglia cell numbers in sham WT, sham KO, WT-TBI, and KO-TBI at 15 and 30 days post-TBI as plotted in Fig. 4f
Total cell numbers in the brain tissue after TBI (Fig. 4b)
1.581 × 106
1.255 × 106
1.408 × 106
1.697 × 106
1.617 × 106
1.986 × 106
Microglia cell numbers in the brain tissue after TBI (Fig. 4f)
Mild TBI does not affect spontaneous locomotor activity or cause excessive anxiety but impairs motor learning and cognitive function
Separate groups of animals (n = 10/group) were evaluated in open-field, elevated plus maze, rota-rod, and Morris water maze 30 days post-TBI.
To examine spontaneous locomotor activity in response to a novel environment, all mice were tested in the open-field behavioral task. The open-field task monitors activity in a brightly lit, novel environment. Spontaneous locomotor activity was assessed as the total amount of distance traveled in the chamber. In addition, anxiety levels can be measured in the open-field task through assessment of the distances traveled in the center versus perimeter of the chamber. This task exploits the natural tendency of mice to avoid open areas.
After open-field and elevated plus maze assessments, the same mice were also tested for coordination and motor skill acquisition using an accelerating rota-rod during each of four trials per day on two consecutive days (Fig. 2b). The amount of time an animal stays on the rota-rod is an indicator of its general level of balance and coordination. In general, mice improve their performance over time with training, which is an indicator of motor learning. Sham wild-type mice performed significantly better than sham CX3CR1−/− mice, during the 2 days of training (Fig. 2b), confirming our previous finding of motor learning deficits in CX3CR1−/− mice . No differences were observed among injury groups during the first day of training. On the second day of training (trials 5–8), sham WT mice remained on the rotor for longer periods. Injured CX3CR1−/− mice performed worse than injured WT. However, the motor impairment of injured CX3CR1−/− mice was similar to that one observed in sham CX3CR1−/−, likely due to the baseline (floor) effect associated with CX3CR1 deficiency. Neither injured CX3CR1−/− mice nor injured WT mice significantly improved motor coordination on the second day of training when compared to day 1 of training; (Fig. 2b; repeated measures ANOVA, p < 0.0001).
Spatial learning was evaluated using the Morris water maze (MWM) task as previously described . As previously shown, the escape latency (time to find the platform) of sham CX3CR1−/− mice during training was longer than that of sham WT mice (Fig. 2c). All mice subjected to TBI, irrespective of genotype, took longer to find the platform during training as compared to sham WT mice. During the probe trial (day 8), the number of target platform crossings was decreased in injured mice irrespective of genotype as compared to sham WT mice. Injured CX3CR1−/− mice performed worse than injured WT and sham mice (Fig. 2d). A similar trend was observed in the time that each group spent in the platform zone (data not shown). There was no difference in the average swim speed among groups (Fig. 2e).
TBI-induced neuronal cell death and survival
Neuronal survival was determined by the stereological quantification of NeuN+ cells (Fig. 3b). At 15 days post-TBI time point, we analyzed tissue from all groups of animals (CX3CR1 sham KO, CX3CR1 sham WT, CX3CR1 KO-TBI, CX3CR1 WT-TBI). However, at 30 days, only tissue from injured TBI animals was analyzed. Neuronal cell loss was apparent after TBI in WT mice and CX3CR1−/− mice (Fig. 3b). In WT mice, there were fewer NeuN+ cells 15 days post-TBI than at 30 days post-TBI. In contrast, there were more NeuN+ cells in CX3CR1−/− mice 15 days post-TBI than at 30 days post-TBI, indicating neuronal cell loss over time. Comparison of genotypes indicated significant differences in numbers of NeuN+ cells such that there were more NeuN+ cells in CX3CR1−/− mice 15 days post-TBI and more NeuN+ cells in WT mice 30 days post-TBI. Collectively, these data demonstrate that CX3CR1 exacerbates cell loss during the acute phase post-TBI (number of neurons is greater in CX3CR1−/− mice 15 days post-TBI than in WT mice) and protects cells during the chronic phase post-TBI (number of neurons is less in CX3CR1−/− mice than WT mice 30 days post-TBI).
CX3CR1 deficiency correlates with increased microglial proliferation and does not modulate the trafficking of hematogenous myeloid cells to the brain after TBI
Impact of mild TBI on microglia phenotypes
CT, ΔCT, and fold change. TGFβ, iNOS, and IL-1β raw numbers as plotted in Fig. 6g. Numbers are expressed as CT, ΔCT, and fold change (means and standard deviations) normalized against wild-type sham
CT (mean ± SD)
ΔCT (mean ± SD)
Fold change (mean ± SD)
25.368 ± 0.31
12.429 ± 0.31
1.000 ± 0.20
25.751 ± 0.23
12.775 ± 0.26
0.782 ± 0.14
25.268 ± 0.03
12.255 ± 0.11
1.107 ± 0.08
25.451 ± 0.32
12.451 ± 0.33
1.154 ± 0.06
29.156 ± 0.11
16.558 ± 0.11
1.000 ± 0.07
29.037 ± 0.12
16.450 ± 0.14
1.080 ± 0.11
28.802 ± 0.24
16.335 ± 0.29
1.187 ± 0.13
29.168 ± 0.06
16.654 ± 0.08
0.933 ± 0.09
28.340 ± 1.01
16.747 ± 099
1.000 ± 0.60
29.705 ± 1.73
17.109 ± 1.72
1.521 ± 0.17
28.903 ± 0.93
16.435 ± 0.90
1.191 ± 0.57
29.259 ± 0.80
16.715 ± 0.80
0.968 ± 0.51
Results of this study demonstrate for the first time that CX3CR1 modulates responses to mild TBI in a time-dependent manner. During the acute post-traumatic period (24 h–15 days), neuronal cell loss is greater in the brain-injured WT than in the brain-injured CX3CR1−/− mice. In contrast, during the chronic phase (30 days post-injury), lack of CX3CR1 exacerbates neuronal damage. The acute post-TBI phase in CX3CR1−/− mice is associated with a protective M2 microglia phenotype as evidenced by upregulation of YM1 and CD206 and increased TGFβ, IL-4, and IL-10. Concurrently with the M2 microglia phenotype during this acute post-traumatic period, responses of CX3CR1−/− mice to TBI include a reduction in M1 microglia phenotype markers, such as iNOS and IL-1β. During the chronic post-injury phase, the brains of injured CX3CR1−/− mice show a profound inflammatory response compared to brains of injured WT mice. This post-traumatic chronic response in mice lacking CX3CR1 is characterized by an inflammatory M1 microglia phenotype, as evidenced by upregulation of Marco, CD68, and the inflammatory cytokines IL-1β and IL-6.
One interpretation for the decrease in TGFβ and IL-4 in the sham KO mice is that lack of CX3CR1 leads to a decrease in the M2-type responses in favor of M1-type responses (increased IL-1β). However, future work will be necessary to test this interpretation and to determine if the polarization of M1-type over M2 type responses is cell autonomous or involves a paracrine signaling mechanism.
The predominant M1 response in injured CX3CR1−/− brain is associated with poorer performance in the Morris water maze. We previously demonstrated that lack of CX3CR1 impairs motor and cognitive function . We now show that lack of CX3CR1 during the chronic phase of mild brain injury exacerbates this already impaired cognitive function. However, it is important to take in consideration that both RT-PCR and ELISA data have been obtained from whole brain tissue, not from extracted microglia; therefore, we cannot determine with absolute certainty the cellular source of these signals. Furthermore, it has to be noted that although the statistical analysis indicated significant difference between CX3CR1 WT-TBI and CX3CR1 KO-TBI in mRNA levels for TGFβ, iNOS, and IL1, the physiological significance of such changes may be questionable and bigger changes may be observed at earlier time points.
Collectively, these observations demonstrate that CX3CR1 is both neuroprotective and neurotoxic in brain injury depending on the time post-injury. Importantly, these findings suggest that antagonizing the CX3CL1/CX3CR1 pathway during a critical period after mild TBI may reprogram the brain inflammatory environment from detrimental to beneficial, favoring endogenous neuroprotective or neurorestorative mechanisms. Furthermore, results from this study provide important new knowledge that is relevant to the differential roles of the CX3CL1/CX3CR1 pathway that have been described in a number of pathologies.
Our findings are in agreement with several reports demonstrating in other models that CX3CR1 drives inflammatory responses during the acute period following CNS injury. For instance, in focal cerebral ischemia, Dénes et al.  reported reduced damage to the blood-brain barrier, which correlated with a protected phenotype, reduced ischemic volume, fewer apoptotic cells, and better performance in the behavioral test of adhesive tape removal. Similarly, CX3CR1−/− mice are protected from the middle cerebral artery occlusion, with smaller infarct size at early time points after injury, which is associated with an M2 microglial phenotype . In a model of spinal cord injury, CX3CR1−/− mice had more favorable outcome 5 days post-injury, with neuroprotection and functional recovery, than WT control mice . The improved recovery after traumatic spinal cord injury is associated with reduced recruitment of monocytes/macrophages to the site of injury. Collectively, these studies underscore a role for CX3CR1 signaling in response to CNS injury and suggest that mechanisms by which CX3CR1 promotes neuroprotection during the acute phase of brain injury include a protective microglia phenotype and reduced monocyte/macrophage recruitment.
Our data indicate that CD68, a specific marker of phagocytic microglia, is decreased at 15 days post-trauma in CX3CR1−/− mice and, in this present study, tends to increase by 30 days post-injury. Although microglial phagocytosis of dead or dying neurons can be beneficial by preventing the release of pro-inflammatory molecules, under some conditions, such as inflammation, microglia also phagocytize viable neurons, thus contributing to cell death . Intraventricular infusion of mesenchymal stem cells after TBI induces early and lasting acquisition of a protective M2 microglial phenotype that is associated with reduced CD68 immunoreactivity . Based on these observations, we hypothesize that the reduced phagocytic activity in CX3CR1−/− mice could be beneficial to neurons and perhaps explain neuroprotection at 15 days post-trauma on these animals. On the other hand, increased phagocytosis 30 days post-TBI could be detrimental and increase neuronal death. However, CD68 immunoreactivity may not necessarily correlate with phagocytosis [47, 48]. For example, microglia that are phagocytizing apoptotic cells in physiological conditions in the hippocampus do not express CD68 [47, 48]. Experiments to specifically address this issue remain to be conducted. Nevertheless, our data show that a transient protective M2 microglia phenotype with decreased CD68 phagocytic activity manifests early after mild TBI in CX3CR1−/− mice.
The dramatic shift during the chronic phase of brain injury in CX3CR1−/− mice limits the recruitment of M2 microglia and switches the balance toward the M1 phenotype, which contributes to excessive inflammatory responses and exacerbated neuronal damage. Although a shift in microglia phenotype between the acute and chronic phase of TBI has recently been reported , we extend these observations by identifying CX3CR1 as a possible regulator of this microglia/macrophage phenotype switch following mild TBI. It is important to note however, that the transition between M1 and M2 microglia phenotypes is not all or none, and several intermediate microglia phenotypes with overlapping features have been described during the shift between alternatively activated phenotypes. Indeed, recently Morganti et al.  using CX3CR1GFP/+ CCR2RFP/+ reporter mice have demonstrated a broad spectrum of M1-M2 polarization mRNA gene expression following TBI, with changes occurring primarily during the first 48 h. Importantly, they reported that there was not a clear delineation of an exclusive M1, M2a, or M2c phenotype. Several factors could explain differences between our study and that of Morganti et al.; ours is a model of mild TBI model, and our M1 and M2 analysis was based on both gene expression and immunohistochemical assessment, whereas the Morganti study only reported on gene expression.
Although our data do not provide information as to precisely how CX3CR1 dictates the recruitment of M1/M2 microglia during acute or chronic stages of the disease, there likely are multiple mechanisms that regulate the neuroprotective vs. neurotoxic effects of microglia after mild TBI. Relative expression of CX3CR1 defines two phenotypically distinct monocyte subsets. Monocytes of the Ly6Chigh/CX3CR1low subset express CCR2, a chemokine receptor that facilitates recruitment of inflammatory monocytes to the site of injury. Conversely, monocytes of the subset Ly6Clow/CX3CR1high are CCR2 deficient and are dependent on CX3CR1 for recruitment [51–53]. CX3CR1+ monocytes may differentiate from Ly6Chigh monocytes in tissue and thus may not require CX3CR1 for recruitment [54, 55]. These distinct monocyte populations are associated with tissue pathology and repair. For example, in a model of myocardial infarction, tissue repair requires recruitment of Ly6Chigh and then Ly6Clow monocytes . Ly6Chigh monocytes are proteolitic, degrade injured tissue, and give rise to M1 macrophages in vivo [35, 56]. Other reports demonstrate that the Ly6Clow/CX3CR1high monocyte subset is associated with beneficial effects as this population is capable of tissue healing .
In experimental models of spinal cord injury and focal cerebral ischemia, tissue damage is attenuated and function recovers earlier following injury in CX3CR1-deficient mice, effects attributed to reduced recruitment and/or activation of microglia/macrophages [23, 24, 57, 58]. Inhibiting CX3CR1 at early time points in a model of ischemic brain injury protects neurological function and reduces neuropathology by decreasing CD11b+/Ly6Clow/iNOS+ monocyte recruitment, microglia proliferation, and leukocyte infiltration . Similarly, Donnelly et al.  showed that abolishing CX3CR1 reduces accumulation of CD11b+/Ly6Clow/iNOS+ monocytes, which confers neuroprotection and promotes recovery of function at early time points after spinal cord injury. These benefits are associated with suppressed inflammatory signaling in microglia and microglia-derived macrophages.
In our model of mild focal TBI, we did not detect differences in leukocyte infiltration from the periphery between wild-type and CX3CR1-deficient mice. Lack of differences between CX3CR1-deficient and wild-type mice could be due to the post-TBI time point analyzed and/or the mild TBI induced in this study. However, based on these aforementioned observations and our data, we hypothesize that after focal TBI, CX3CR1 plays a fundamental role in dictating the timing of recruitment of inflammatory monocyte populations relative to the protective monocyte populations, which results in a detrimental and protective microglia phenotype, respectively. Our results may reconcile data obtained from a number of pathologies with respect to whether CX3CR1 is protective or neurotoxic. Taken together, our data suggest that CX3CR1 may be an ideal target for therapeutic intervention in the acute post-traumatic period to delay secondary brain damage.
This study was supported by the Department of Anesthesiology and Pain Medicine of the University of Washington and by NIH SC1GM095426.
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