Synaptic loss and progression in mice infected with Angiostrongylus cantonensis in the early stage
Journal of Neuroinflammation volume 19, Article number: 85 (2022)
Angiostrongylus cantonensis is also known as rat lungworm. Infection with this parasite is a zoonosis that can cause eosinophilic meningitis and/or eosinophilic meningoencephalitis in humans and may lead to fatal outcomes in severe cases. In this study, we explored the mechanisms of the impairments in the cognitive functions of mice infected with A. cantonensis.
In infected mice with different infective intensities at different timepoint postinfection, loss and recovery of cognitive functions such as learning and memory abilities were determined. Neuronal death and damage to synaptic structures were analyzed by Western blotting and IHC in infected mice with different infection intensities at different timepoint postinfection.
The results of behavioral tests, pathological examinations, and Golgi staining showed that nerve damage caused by infection in mice occurred earlier than pathological changes of the brain. BDNF was expressed on 14 day post-infection. Cleaved caspase-3 increased significantly in the late stage of infection. However, IHC on NeuN indicated that no significant changes in the number of neurons were found between the infected and uninfected groups.
The synaptic loss caused by the infection of A. cantonensis provides a possible explanation for the impairment of cognitive functions in mice. The loss of cognitive functions may occur before severe immunological and pathological changes in the infected host.
Angiostrongylus cantonensis is the most important causative agent of eosinophilic meningoencephalitis in humans [1, 2]. The first documented human infection was recognized in Taiwan (Tainan Hospital) in 1944 after an autopsy of a 15-year-old boy who died of suspected eosinophilic meningoencephalitis. Approximately 10 live immature worms were discovered from the patient’s cerebrospinal fluid .
Although infected people with angiostrongyliasis are commonly recognized in Southeast Asia, the Caribbean, the Pacific Basin, and especially in China, the infection was recently found to be an emerging infectious disease and widespread throughout the world. By 2010, more than 2900 cases had been reported in approximately 30 countries [4,5,6]. Humans are a nonpermissive host of A. cantonensis. The infection is due to ingestion of raw or uncooked intermediate hosts (land snails, freshwater snails, or slugs) or paratenic hosts (crustaceans, frogs, fishes, or planarians) contaminated with infective third-stage larvae. People may also acquire infection by consuming vegetables polluted with infective larvae in the mucus trail of infected gastropods .
The brain is the important organ responsible for cognitive functions, including learning and memory formation. Brain lesions caused by internal interferences such as drugs, thrombosis, or invasions of pathogens may affect learning and memory [8, 9]. Parasitic infections have been observed to change the behavior of their hosts and facilitate their transmission and completion of life history. The fish Rutilus lacustris living in deep waters changes its habitat to shallow waters near the shore after infection with Ligula intestinalis. This behavior increases the chance of predation of the fish by waterfowl and enables the spread of the parasite to its avian final hosts . Infection of bees with Nosema ceranae stimulates prematurity in development. The flight activity of premature bees is higher than that of normal bees, and the success rate of homing is decreased. However, these behavioral changes do not have a direct relation to the life history of the parasite .
In addition to behavioral changes, the memory of the host may also be affected by parasitic infections. Testing mice infected with three different intensities of Toxocara canis (T. canis) larvae in T-maze, water maze, and elevated plus maze assessments demonstrated poor spatial learning and memory in the high-dose group . Using the water maze to determine spatial learning and memory ability, Toxoplasma gondii (T. gondii) infected mice were found to have significantly longer training days, swimming times, and swimming distances than their uninfected counterparts. These findings confirm that T. gondii infection affects spatial learning in mice .
Zhang et al. employed flow cytometry to analyze brain cells in BALB/c mice infected with A. cantonensis. Necrosis and apoptosis of astrocytes and microglia were found in brain tissue 21 days after infection, but did not cause neuronal death. However, the results of the water maze showed significant decreases in the spatial learning and memory abilities of the infected mice on day 14 postinfection . Although A. cantonensis infection did not cause the death of neurons, it significantly affected the cognitive functions regulated by the neurons. Therefore, this study intended to explore the mechanisms of spatial learning and memory impairment in mice infected with A. cantonensis. Considering that BALB/c mice were susceptible to A. cantonensis, C57BL/6 mice with higher tolerance should be used to avoid affecting the accuracy of behavior-related tests due to mobility [15,16,17].
Materials and methods
Maintenance of the life cycle of A. cantonensis
By the modified Baermann filtration method, first-stage larvae were isolated from the feces of infected rats before infection with B. glabrata snails. On day 21, the infected snails were sacrificed, and third-stage larvae were recovered from snail tissues minced with a homogenizer (Cole-Parmer Instrument Co., USA). After digestion with artificial gastric juice (0.6% w/v pepsin, pH 2–3) at 37 °C for 45 min, the third-stage larvae were isolated and then inoculated into SD rats. On day 50 postinfection, first-stage larvae will be isolated from the feces of the infected rats.
For A. cantonensis life cycle maintenance, SD rats were purchased from the National Laboratory Animal Center (Taipei) or BioLASCO (Taipei). For experimental studies, C57BL/6 mice (7–8 weeks) were purchased from the National Laboratory Animal Center (Taipei) or BioLASCO (Taipei). These animals were cared for in the Animal Center of Chang Gung University. They were reared at a constant temperature and provided with food and drinking water ad libitum. All procedures involving animals and their care were reviewed and approved by the Chang Gung University Institutional Animal Care and Use Committee (IACUC Approval No.: CGU108-208).
After isolation from B. glabrata snails by the above method, the third-stage larvae were counted and removed by a glass capillary under a dissecting microscope. Each mouse was inoculated with 50 larvae by stomach intubation using a feeding tube.
This study was to investigate the changes in cognitive function of mice at specific timepoints after infection. The mice were divided into MWM group and NOR + Y-maze group. The Y-maze test was employed to test the effect of angiostrongyliasis on the spatial working memory performance of mice. There were 10 mice in each of the control group and the infection groups. A total of 40 mice were used in this experiment. The device consists of custom-made Y-mazes with three symmetrical and identical arms (15 × 5 × 40 cm). The tested mouse was placed at the end of a randomly assigned arm and allowed to freely navigate in the maze for 5 min. The sequence and number of arm entries were recorded using a video camera throughout the maze. The sequence triads in which all three arms are explored (including ABC, ACB, BAC, BCA, CAB, and CBA) are considered successful alternations with normal perception and short-term memory. The percentage of successful alterations is calculated as the number of successful alterations divided by the number of possible alternations (the total number of arm entries − 2). The total number of arm entries serves as an indicator of locomotor activity .
Morris water maze test
The Morris water maze (MWM) test was used to assess the effect of angiostrongyliasis on spatial memory. There were 10 mice in each of the control group and the infection groups. A total of 40 mice were used in this experiment. The custom-made maze is a circular pool 120 cm in diameter and 40 cm in height filled with water at 25 ± 1 °C that is made opaque with the addition of milk. In the acquisition session, each mouse was given four trials per day and 4 days of training in total to find a hidden platform located 1.5 cm below the water surface. Each mouse was placed into the pool facing the wall, with a different starting point for each trial in which the direct route to the platform differed each time. The time required by the mouse to find and stand on the platform was recorded for up to 90 s. The mouse was allowed to stay on the platform for 30 s. It was then removed from the maze and placed in its cage. If the mouse did not find the platform within 90 s, the animal was placed on the platform for 30 s. The intertrial interval was at least 30 min. In the probe session, on day 5, the platform was removed from the pool. The mouse was tested in a probe trial for 60 s. Mouse swimming tracks were recorded using a TopScan automated tracking system (Clever Sys Inc., Virginia, USA) .
Novel object recognition task
The novel object recognition (NOR) task was used to evaluate the effect of angiostrongyliasis on recognition memory. There were 10 mice in each of the control group and the infection groups. A total of 40 mice were used in this experiment. The NOR task consisted of three types of sessions: habituation, training, and testing. For habituation, the tested mouse was placed in a box for 10 min in the absence of test objects once per day for three consecutive days. On the fourth day, two identical objects were placed symmetrically on two opposite sides of the box. The mouse was allowed to explore the box for 10 min in the training session. Approximately 24 h after the training session, the mouse was allowed to explore the same box. However, one familiar object was replaced by a novel object. The animals were then allowed to explore freely for 10 min. The times spent exploring familiar or novel objects are recorded. Data were compiled up to 40 s of total exploration time. Object exploration was defined as a mouse touching or sniffing the object at a distance less than or equal to 2 cm. The discrimination index (DI) refers to the difference in exploration time for a novel and a familiar object divided by the amount of exploration of the novel and familiar objects [DI = (novel − familiar)/(novel + familiar)] .
Collection of blood and brain specimens
From each mouse, blood samples were collected by cardiac puncture after complete anesthetization with isoflurane. The brain is then removed from the cranial cavity. Serum specimens were prepared from the blood samples by centrifugation and kept at − 80°. The brain is stored in 10% formalin solution.
Preparation of sera
After cardiac puncture, blood samples were placed at room temperature for 2 h and then at 4 °C overnight. After centrifugation at 1300×g for 45 min, the supernatant was collected and kept at − 80 °C.
After complete fixation in 10% formalin solution, each brain was cut into five sections: anterior cerebrum, lateral ventricles, third ventricle and hippocampus, posterior cerebrum, and fourth ventricle. These sections were then dehydrated with alcohol, embedded in paraffin, and cut into slices.
There were 5 mice in each of the control group and the infection groups. Brain tissues were homogenized in ice-cold 1% SDS and protein concentrations were determined in triplicates using the Bradford protein assay. The extracts were mixed with Laemmli sample buffer and placed in a boiling water bath for 5 min. Proteins were separated by SDS–PAGE and blotted onto nitrocellulose membranes. Designated protein species (BDNF; cleaved caspase-3 for apoptosis; Beclin-1 for autophagy; RIP3 for necroptosis) (Abcam, ab108319, UK; Cell Signaling Technology, 9664, USA; Abcam, ab62557, UK; Abcam, ab56164, UK) were detected using their corresponding specific primary antibodies followed by horseradish peroxidase conjugated secondary antibodies. Protein immunoreactive signals are detected with the ChemiDocTM XRS + System (Bio-Rad, USA).
Enzyme-linked immunosorbent assay
After coating wells of ELISA microplates with the somatic antigen preparation from the fifth-stage larvae at 4 °C overnight, the wells were then washed with a blocking buffer before adding serum specimens (containing the primary antibodies) from C57BL/6 mice sacrificed at different timepoints. There were 5 mice in each of the control group and the infection groups. The excess amounts were removed by washing and secondary antibodies (goat anti-mouse IgA/G/M; goat anti-mouse IgG; goat anti-mouse IgM) (KPL, 07511807, USA; Sigma-Aldrich, A3562, USA; KPL, 07511803, USA) conjugated with alkaline phosphatase were then added. Color was then developed after adding a substrate and reactions were terminated by adding a stop solution. Readings were measured at OD 405 nm.
Deeply anesthetized mice were transcardially perfused with PBS followed by 4% paraformaldehyde in PBS. Fixed brains were sliced into 30 µm sections. There were 5 mice in each of the control group and the infection groups. PSD95, SYN, NeuN (Cell Signaling Technology, 3409, USA; Abcam, ab32127, UK; Abcam, ab128886, UK) were stained in each of the five brain sections from each animal. Image J was used to threshold the stained brain tissue and to analyze the color ratio of the fixed area in the imaging.
Hematoxylin and eosin staining
Each brain section was sliced into 3 µm sections and treated with xylene to remove paraffin and then stained with Mayer’s hematoxylin solution and eosin solution. The slice was mounted on a microscopic slide using mounting medium.
Regions of the mouse brain were divided into anterior cerebrum, lateral ventricles, third ventricle and hippocampus, posterior cerebrum and fourth ventricle, and cerebellum. Eight pathological changes were employed to determine the degree of brain damage post-infection. These changes included eosinophilic meningitis, brain necrosis, choriomeningitis, size/finding of larvae, meninges congestion, perivascular cuffing, encephalitis, and hemorrhage. After determining the area of each change in the slices, medians were calculated and employed as a cutoff point for severity. Negative results were scored 0, scores of 1 represented those below or equal to the median, and scores of 2 indicated those above the median. A score of severity for each pathological change was calculated by each group of mice .
After removal from the cranial cavities of the mice, the brains were quickly removed and prepared for Golgi impregnation using the FD Rapid Golgi Stain Kit (FD Neurotechnologies, Baltimore, MD). There were 5 mice in each of the control group and the infection groups. After incubating in Golgi-Cox for 17 days at room temperature in the dark, the brains received solution C at 4 °C for 3 days before embedding in OCT and storage at − 80 °C. sections (150 µm) were transferred into the staining solution for 10 min before dehydration, cleaning in xylene, and coverslipping with DPX mounting medium (VWR International, Leuven, Belgium). Images were captured on an Olympus BX53 microscope (100X magnification). Spines from granular neurons were counted and normalized against the total dendritic length.
For the neuronal apoptosis assay, frozen brain sections were stained using the terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) kit (AP 11684809910; Roche Life Science, Switzerland) to detect DNA fragmentation. There were 5 mice in each of the control group and the infection groups. We used image J to threshold the stained brain tissue and to analyze the color ratio of the fixed area in the imaging.
Data are expressed as the mean ± standard deviation. Differences among the groups were analyzed by one-way ANOVA, one-way repeated measures ANOVA, two-way ANOVA, and two-way repeated measures ANOVA.
Behavioral test of C57BL/6 mice infected with A. cantonensis
The Morris water maze was divided into an acquisition test (spatial learning) on the first 4 days and a probe test (spatial memory) on the 5th day (Fig. 1a). In the acquisition test, the time for mice to reach the platform decreased with the increase in the number of learning days. The spatial learning ability of C57BL/6 mice was significantly reduced on day 14 postinfection (Fig. 1b). In the probe test, the time that C57BL/6 mice stayed in the target quadrant decreased with the increase in the number of days of infection, indicating that the spatial memory ability of the mice was affected by the infection, and it decreased significantly on day 14 postinfection (Fig. 1c).
NOR task was used to evaluate the ability of mice to recognize new things before and after infection with A. cantonensis. The results showed that the ability of the mice to recognize new things was significantly reduced on day 14 postinfection (Fig. 1d). The Y maze was used to explore the effect of A. cantonensis infection on the spatial working memory of C57BL/6 mice. The number of infected mice entering the arms was significantly reduced on day 14 postinfection (Fig. 1e). There was no significant change in the working memory ability of infected mice (Fig. 1f). The open field test results showed that A. cantonensis infection did not affect the mobility of the mice (Additional file 1: Fig. S1).
In summary, cognitive function related to the long-term memory of mice was impaired in the early stages of infection. In the Y maze, it was found that A. cantonensis infection may have an effect on the desire to explore, but it has no effect on short-term memory ability.
Changes in nerve structure in C57BL/6 mice infected with A. cantonensis
Dendrite damage in the hippocampal neurons of infected mice was detected by Golgi staining. The nerve distribution in the hippocampus of C57BL/6 mice tended to decrease with increasing days of infection (Fig. 2a). The total number of dendritic ring intersections decreased as the infection time increased. The distribution of dendritic intersections was observed; the reduced part was mainly located on the side close to the neuron, and the distal end only decreased significantly in the late stage of infection. Dendritic intersections represent the complexity of nerve structure and the dynamic range of nerves (Fig. 2b). The length of dendrites decreased significantly on day 7 postinfection (Fig. 2c). Dendritic segments showed the spine morphology in infected mice (Fig. 2d). The dendritic spine density significantly decreased on day 7 postinfection (Fig. 2e, f). The nerve dendrite structure of infected mice was damaged in the early stages of infection.
Effect of A. cantonensis infection on the expression of synaptic proteins in C57BL/6 mouse brains
Changes in the expression levels of PSD95, synaptophysin, and NeuN in the hippocampus of mice infected with A. cantonensis over time were determined by immunohistochemistry. The levels of PSD95 and synaptophysin significantly decreased on day 7 postinfection (Fig. 3d, f). NeuN is specific to the neuronal cell nucleus, so it is used to label neuronal cells. There was no significant decrease in NeuN expression at different timepoints after infection (Fig. 3b). These findings indicated that A. cantonensis infection may cause damage to nerve synapses but does not cause the death of neurons. The levels of PSD95, synaptophysin and NeuN expression in the hippocampus of mice infected with A. cantonensis were detected by Western blotting (Fig. 4a). The expression levels of PSD95 and synaptophysin decreased significantly after infection (Fig. 4c, d), while NeuN expression was not affected by A. cantonenesis infection (Fig. 4e). Then, we evaluated the expression levels of PSD95, synaptophysin and NeuN in the prefrontal cortex (Fig. 4b). The prefrontal cortex is also the functional area of the brain that regulates cognition. The expression levels of PSD95 and synaptophysin decreased significantly after infection (Fig. 4f, g), while NeuN expression was not affected by the infection (Fig. 4h) (Additional file 2: Fig. S2).
Brain pathological changes and survival rates of C57BL/6 mice infected with A. cantonensis
Pathological changes (eosinophilic meningitis, hemorrhage, larvae, encephalitis) in the brain and survival rates of infected mice were compared at different timepoints (Fig. 5). Infected mice died starting on day 23 postinfection (Fig. 5a). The pathological findings of infected mice had higher scores on day 14 postinfection (Fig. 5b, c). After day 21, the severity of encephalitis increased in the infected mice (Fig. 5d). Scoring of the presence of larvae in the cerebral parenchyma of infected mice was higher on day 21 postinfection (Fig. 5e). The results demonstrated that pathological changes mainly appeared in the late stage of A. cantonenesis infection, and there was no serious inflammatory reaction in the early stage of infection.
We wanted to know whether the migration of the larvae in the brain caused mechanical damage to the nerve structure. The location of larvae in the brain at different timepoints after infection was estimated (Fig. 6). The larvae were mainly distributed in the first area of the brain section containing the olfactory bulb and the prefrontal cortex on day 7 postinfection (Fig. 6a), and the larvae were mainly distributed in the second area of the brain section containing the striatum on day 14 postinfection (Fig. 6b). On day 21 postinfection, the larvae migrated to the back of the brain and were active in the third, fourth, and fifth areas of the brain section, including the hippocampus and cerebellum (Fig. 6c). There was no difference in the recovery rates of larvae at different timepoints after infection (Fig. 6d). The larvae grew in length as the number of days of infection increased, and the length increased significantly on day 12 postinfection (Fig. 6e). The results showed that A. cantonensis infection caused the impairment of cognitive function and the destruction of synaptic structure in the early stage of infection, but the larvae had not yet migrated to the hippocampus and caused mechanical damage.
Expression of cell death-related proteins in the brains of C57BL/6 mice infected with A. cantonensis
The expression levels of BDNF, cleaved caspase-3, RIP3, and Beclin-1 in the hippocampus of mice infected with A. cantonensis over time were determined by Western blotting. The expression of BDNF significantly increased on day 14 postinfection (Fig. 7a), and the expression of cleaved caspase-3 significantly increased on day 21 (Fig. 7b).
The TUNEL assay was used to confirm cell death in the brains of mice before and after infection. Cell death increased significantly on day 21 postinfection (Fig. 7e, f). The results showed that the nerve structure damage and cognitive dysfunction caused by A. cantonensis infection in the early stage were not caused by cell death.
Peripheral immune response of C57BL/6 mice infected with A. cantonensis
The expression of peripheral immunoglobulin in the serum of C57BL/6 mice infected with A. cantonensis was detected by ELISA. IgM increased significantly on day 7 postinfection but gradually slowed down on day 14 postinfection (Fig. 8a). IgG increased significantly on day 14 postinfection (Fig. 8b). IgA + G + M levels had increased significantly on day 7 postinfection and continued to increase as the number of days of infection increased (Fig. 8c). In the early stage of A. cantonensis infection, the peripheral immunity of the mouse is expressed by IgM. As the infection time increased, it was highly expressed by IgG in the late stage (Fig. 8d).
The water maze was used to evaluate the spatial learning and memory abilities of C57BL/6 mice infected with A. cantonensis. The results showed that the spatial learning and memory abilities of the mice were impaired on day 14 postinfection. The NOR task and Y maze were used to test the spatial memory and working memory abilities of the infected mice. The NOR task results showed that the memory ability of the mice decreased significantly on day 14 postinfection. However, the Y maze test showed that there were no significant differences between the mice before and after infection. These findings indicate that A. cantonensis infection may not have much impact on working memory. A. cantonensis larvae invade the host's brain and mainly migrate between blood vessels and ventricles. Around the hippocampus is the third ventricle, and the prefrontal cortex is the brain parenchyma area. Therefore, it can be explained that the damage to the prefrontal cortex of the brain is lower than that of the hippocampus, and the impact on working memory is also relatively slight.
The survival rate and brain pathology of C57BL/6 mice were also analyzed in this study. The mice began to die on day 23 postinfection. Eight pathological changes were analyzed, including meningitis, congestion, hemorrhage, larval findings, choroid plexitis, perivascular cuffing, encephalitis, and brain necrosis. The overall pathological changes increased on day 14 postinfection. Analysis of encephalitis showed a significant increase on day 21 postinfection. Previous studies have also found that the larvae moved from the forebrain to the cerebellum with the time of infection. The larvae mostly appeared in the cavity rather than the brain parenchyma.
The density of dendritic spines was positively correlated with the formation of memory. An increased density of dendritic spines may strengthen the formation of memory. Aging or Alzheimer’s disease may cause a reduction in memory by decreasing the density of dendritic spines . Moreover, spatial memory and working memory have also been related to the density of dendritic spines . Golgi staining analysis of the hippocampus of the mice after behavioral tests showed that the density of dendritic spines on the nerve synapse decreased significantly on day 7 postinfection. The number of dendrite-ring intersections also decreased with the time of infection. A. cantonensis caused synaptic loss in the early stages of infection. The results of this study showed that the dendritic structure of the hippocampus began to be damaged in the anterior and middle segment of the mouse in the initial stage of infection, but the distribution of the posterior dendrites has not been affected. As the infection time increased, the overall structure of the dendrites was severely damaged.
Rats subjected to water maze training induced the rapid recruitment of NMDAR subunits and PSD95 to synaptic lipid rafts, which were associated with the formation of memory . Environmental enrichment could be a potent cognitive enhancer for aged females, the mechanism of which was to enhance spatial memory and increase synaptophysin levels in the hippocampus and frontoparietal cortex . IHC staining was used to analyze synaptic proteins in the mouse brain and the neuron marker NeuN. The synaptic proteins PSD95 and synaptophysin showed significant decreases on day 7 postinfection, while NeuN showed no significant difference before and after infection in mice. These findings suggest that A. cantonensis infection may cause damage to nerve synapses but does not cause the death of neurons. Moreover, A. cantonensis caused synaptic loss in the early stage of infection, which in turn reduced cognitive functions.
Synaptic loss is currently considered to be the most relevant factor for cognitive decline in neurodegenerative diseases, such as Alzheimer's disease (AD). Antibodies against the synaptic terminals—synapsin-1 and synaptophysin—are used as synaptic markers in the hippocampal complexes of AD patients and non-dementia control subjects. Compared with the control brain, all AD patients had significantly less synaptic staining in the outer half of the molecular layer of the dentate gyrus. In addition, it has also been found that synaptic loss is an early event in the disease process. Intrinsic factors (such as elevated calcium ions) and external factors (such as amyloid or Tau pathology-induced microglia phenotype transformation) can destroy the stability of dendritic spines and may particularly cause synaptic loss [24,25,26]. Studies have pointed out that neuroinflammation and excitotoxic damage will also reduce synaptic markers in the rat brain .
Previous studies have found that the excretory/secretory products (ESPs) of fifth-stage larvae of A. cantonensis can prompt mouse brain astrocytes to activate the Sonic hedgehog (Shh) pathway, reduce oxidative stress, induce autophagy, and inhibit cell apoptosis [28, 29]. Through the activated Shh pathway, the expression of nuclear factor-κB (NF-κB) was increased. The function of NF-κB is to regulate inflammation and promote the secretion of proinflammatory genes, including cytokines and chemokines, such as IL-1β and TNF-α. The ESP of fifth-stage larvae of A. cantonensis stimulated the activation of mouse brain astrocytes and the production of inflammation-related cytokines through the Shh pathway and NF-κB [30,31,32].
Infection with A. cantonensis caused damage to the nerve structure in the early stage of infection and affected cognitive function. Early infected larvae did not appear around the hippocampus. The symptoms of brain inflammation did not increase significantly until 21 days after infection. The results indicate that the larvae have related factors (e.g., excretory/secretory product, ESP) that may cause the loss of synapses and cognitive function at the initial stage of infection, rather than mechanical damage and inflammatory pathology.
Gosnell WL et al. suggested the role of eosinophils in angiostrongyliasis. Eosinophils have also been shown to store, produce, and release neurotrophins, such as BDNF. It has been suggested that locally produced BDNF in the CNS mitigates inflammation-dependent neuronal damage . Studies have pointed out that traumatic brain injury (TBI) can activate the BDNF/TrkB pathway to cause changes in expression of BDNF mRNA . The results of clinical study showed that in the CSF of TBI patients, there was a higher expression of BDNF . The infection of A. cantonensis cause eosinophilic meningoencephalitis, and a large amount of eosinophil infiltration may also increase the expression of BDNF. Western blotting was used to analyze the expression levels of the mouse brain neurotrophic factor BDNF, the cell death-related apoptosis factor cleaved caspase-3, the necroptosis factor RIP3, and the autophagy factor Beclin-1. The results showed that the brains of mice were damaged on day 14 postinfection and that BDNF increased significantly. Regarding cell death-related factors, only the apoptotic factor cleaved caspase-3 increased significantly on day 21 postinfection, while the necroptosis factor RIP3 and the autophagy factor Beclin-1 showed no significant changes. The results showed that the damage to neural structures and cognitive functions caused by A. cantonensis early infection was not caused by cell death.
Many studies have pointed out that immune-related cytokines/chemokines can affect cognitive function. Kipnis et al. injected T cells into nude mice, and their spatial memory learning ability was significantly improved . Derecki et al. conducted a water maze test with C57BL/6 mice that had been knocked out of the IL-4 gene and found that their spatial memory learning ability was decreased . In addition to the peripheral immune factors that affect the brain, microglia and astrocytes are widely discussed in neurodegenerative diseases. It is generally believed that the abnormal effects of microglia and astrocytes will cause abnormal protein accumulation and increase in oxidative stress, which in turn leads to damage to nerve structure and affects cognitive function . Angiostrongyliasis is known to activate microglia and increase oxidative stress in the brain. The results of this research indicate that the excretory/secretory products of larvae may cause synaptic loss to affect cognitive function. The relationship between A. cantonensis infection and neurodegenerative diseases is a subject worthy of study.
In this study, it was found that A. cantonensis caused synaptic loss in the early stage of infection. The infection affects the cognitive functions of the host and did not cause the death of neurons. Damage to nerve structure was not caused by the mechanical destruction of larval migration or pathological changes. Therefore, it was speculated that the larvae may cause synaptic loss through the ESP, thereby affecting the learning and memory abilities of the host. It is anticipated that the influence of synaptic loss caused by the infection of A. cantonensis may provide another possible explanation for the loss of cognitive functions postinfection.
Availability of data and materials
The data obtained from this study are included in this published article.
Analysis of variance
Brain-Derived Neurotrophic Factor
Central nervous system
- IgA + G + M:
Immunoglobulin A + G + M
Morris water maze
Novel object recognition
Postsynaptic density protein 95
Receptor-interacting protein 3
Traumatic brain injury
Tumor necrosis factor-α
Tropomyosin receptor kinase B
Alicata JE. Historical summary of Angiostrongyliasis cantonensis as a new parasitic disease of man. Bull Soc Pathol Exot Filiales. 1988;81:766–8.
Alicata JE. The discovery of Angiostrongylus cantonensis as a cause of human eosinophilic meningitis. Parasitol Today. 1991;7:151–3.
Nomura S, Lin H. First clinical case of Haemostrongylus ratti (Japennnese text). Taiwan No Ikai. 1945;3:589–92.
Wang QP, Lai DH, Zhu XQ, Chen XG, Lun ZR. Human angiostrongyliasis. Lancet Infect Dis. 2008;8:621–30.
Wang QP, Wu ZD, Wei J, Owen RL, Lun ZR. Human Angiostrongylus cantonensis: an update. Eur J Clin Microbiol Infect Dis. 2012;31:389–95.
Barratt J, Chan D, Sandaradura I, Malik R, Spielman D, Lee R, Marriott D, Harkness J, Ellis J, Stark D. Angiostrongylus cantonensis: a review of its distribution, molecular biology and clinical significance as a human pathogen. Parasitology. 2016;143:1087–118.
Kramer KJ, Posner J, Gosnell WL. Role of gastropod mucus in the transmission of Angiostrongylus cantonensis, a potentially serious neurological infection. ACS Chem Neurosci. 2018;9:629–32.
Mahncke HW, Bronstone A, Merzenich MM. Brain plasticity and functional losses in the aged: scientific bases for a novel intervention. Prog Brain Res. 2006;157:81–109.
Schultz W. Neuronal reward and decision signals: from theories to data. Physiol Rev. 2015;95:853–951.
Loot G, Brosse S, Lek S, Guégan JF. Behaviour of roach (Rutilus rutilus L.) altered by Ligula intestinalis (Cestoda: Pseudophyllidea): a field demonstration. Freshwater Biol. 2001;46:1219–27.
Paris L, El Alaoui H, Delbac F, Diogon M. Effects of the gut parasite Nosema ceranae on honey bee physiology and behavior. Curr Opin Insect Sci. 2018;26:149–54.
Cox DM, Holland CV. Relationship between three intensity levels of Toxocara canis larvae in the brain and effects on exploration, anxiety, learning and memory in the murine host. J Helminthol. 2001;75:33–41.
Wang HL, Bao AY, Wang GH, Jiang MS, Liu ZC, Dong HF, Guo Y. Effect of chronic Toxoplasma infection on the spatial learning and memory capability in mice. Zhongguo Ji Sheng Chong Xue Yu Ji Sheng Chong Bing Za Zhi. 2006;24:114–8.
Zhang MY, Xu YY, Pan T, Hu Y, Limpanont Y, Huang P, Okanurak K, Wu YQ, Dekumyoy P, Zhou HG, Watthanakulpanich D, Wu ZD, Wang Z, Lv ZY. Apoptosis and necroptosis of mouse hippocampal and parenchymal astrocytes, microglia and neurons caused by Angiostrongylus cantonensis infection. Parasit Vectors. 2017;10:611.
Chen X, Oppenheim JJ, Howard OM. BALB/c mice have more CD4+CD25+ T regulatory cells and show greater susceptibility to suppression of their CD4+CD25- responder T cells than C57BL/6 mice. J Leukoc Biol. 2005;78:114–21.
Jovicic N, Jeftic I, Jovanovic I, Radosavljevic G, Arsenijevic N, Lukic ML, Pejnovic N. Differential immunometabolic phenotype in Th1 and Th2 dominant mouse strains in response to high-fat feeding. PLoS ONE. 2015;10:e0134089.
Wang TY, Chen KY, Jhan KY, Li CH, Jung SM, Wang LC. Temporal-spatial expressions of interleukin-4, interleukin-10, and interleukin-13 in the brains of C57BL/6 and BALB/c mice infected with Angiostrongylus cantonensis: an immunohistochemical study. J Microbiol Immunol Infect. 2020;53:592–603.
Chang PK, Yu L, Chen JC. Dopamine D3 receptor blockade rescues hyper-dopamine activity-induced deficit in novel object recognition memory. Neuropharmacology. 2018;133:216–23.
Jhan KY, Cheng CJ, Jung SM, Lai YJ, Chen KY, Wang LC. Co-therapy of albendazole and dexamethasone reduces pathological changes in the cerebral parenchyma of Th-1 and Th-2 dominant mice heavily infected with Angiostrongylus cantonensis: histopathological and RNA-seq analyses. Biomolecules. 2021;11:536.
Frankfurt M, Luine V. The evolving role of dendritic spines and memory: interaction(s) with estradiol. Horm Behav. 2015;74:28–36.
Mahmmoud RR, Sase S, Aher YD, Sase A, Gröger M, Mokhtar M, Höger H, Lubec G. Spatial and working memory is linked to spine density and mushroom Spines. PLoS ONE. 2015;10:e0139739.
Delint-Ramírez I, Salcedo-Tello P, Bermudez-Rattoni F. Spatial memory formation induces recruitment of NMDA receptor and PSD-95 to synaptic lipid rafts. J Neurochem. 2008;106:1658–68.
Frick KM, Fernandez SM. Enrichment enhances spatial memory and increases synaptophysin levels in aged female mice. Neurobiol Aging. 2003;24:615–26.
Hamos JE, DeGennaro LJ, Drachman DA. Synaptic loss in Alzheimer’s disease and other dementias. Neurology. 1989;39:355–61.
Scheff SW, Price DA, Schmitt FA, Mufson EJ. Hippocampal synaptic loss in early Alzheimer’s disease and mild cognitive impairment. Neurobiol Aging. 2006;27:1372–84.
Subramanian J, Savage JC, Tremblay MÈ. Synaptic loss in Alzheimer’s Disease: echanistic Insights Provided by Two-Photon in vivo Imaging of Transgenic Mouse Models. Front Cell Neurosci. 2020;14:592607.
Rao JS, Kellom M, Kim HW, Rapoport SI. Neuroinflammation and synaptic loss. Neurochem Res. 2012;37:903–10.
Chen KY, Cheng CJ, Wang LC. Activation of sonic hedgehog leads to survival enhancement of astrocytes via the GRP78-dependent pathway in mice infected with Angiostrongylus cantonensis. Biomed Res Int. 2015;2015:674371.
Chen KY, Chiu CH, Wang LC. Anti-apoptotic effects of Sonic hedgehog signalling through oxidative stress reduction in astrocytes co-cultured with excretory-secretory products of larval Angiostrongylus cantonensis. Sci Rep. 2017;7:41574.
Chen KY, Wang LC. Stimulation of IL-1β and IL-6 through NF-κB and sonic hedgehog-dependent pathways in mouse astrocytes by excretory/secretory products of fifth-stage larval Angiostrongylus cantonensis. Parasit Vectors. 2017;10:445.
Chen KY, Cheng CJ, Cheng CC, Jhan KY, Chen YJ, Wang LC. The excretory/secretory products of fifth-stage larval Angiostrongylus cantonensis induces autophagy via the Sonic hedgehog pathway in mouse brain astrocytes. PLoS Negl Trop Dis. 2020;14:e0008290.
Chen KY, Chen YJ, Cheng CJ, Jhan KY, Wang LC. Excretory/secretory products of Angiostrongylus cantonensis fifth-stage larvae induce endoplasmic reticulum stress via the Sonic hedgehog pathway in mouse astrocytes. Parasit Vectors. 2020;13:317.
Gosnell WL, Kramer KJ. The role of eosinophils in angiostrongyliasis: multiple roles for a versatile cell? Hawaii J Med Public Health. 2013;72:49–51.
Gustafsson D, Klang A, Thams S, Rostami E. The role of BDNF in experimental and clinical traumatic brain injury. Int J Mol Sci. 2021;22:3582.
Failla MD, Conley YP, Wagner AK. Brain-derived neurotrophic factor in traumatic brain injury-related mortality: interrelationships between genetics and acute systemic and central nervous system BDNF profiles. Neurorehabil Neural Repair. 2016;30:83–93.
Kipnis J, Cohen H, Cardon M, Ziv Y, Schwartz M. T cell deficiency leads to cognitive dysfunction: implications for therapeutic vaccination for schizophrenia and other psychiatric conditions. PNAS. 2004;101:8180–5.
Derecki NC, Cardani AN, Yang CH, Quinnies KM, Crihfield A, Lynch K, Kipnis J. Regulation of learning and memory by meningeal immunity: a key role for IL-4. J Exp Med. 2009;207:1067–80.
Chen L, Pan H, Bai Y, Li H, Yang W, Lin ZX, Cui W, Xian YF. Gelsemine, a natural alkaloid extracted from Gelsemium elegans Benth alleviates neuroinflammation and cognitive impairments in Aβ oligomer-treated mice. Psychopharmacology. 2020;237:2111–24.
We would like to thank Yi-Tang Lin for technical support.
This study was supported in part by grants from the Ministry of Science and Technology, Taiwan (Grant No. MOST109-2320-B-182-032-MY3), and the Chang Gung Memorial Hospital (Grant No. CMRPD1H0343 and CMRPD1H0443).
Ethics approval and consent to participate
Laboratory animal applications in this study were approved by the Institutional Animal Care and Use Committee, Chang Gung University (IACUC Approval No.: CGU108-208).
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The authors declare that they have no competing interests.
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Evaluation of mobility of C57BL/6 mice before and after Angiostrongylus cantonensis infection by open field tests. a The total distance of C57BL/6 mice moved in open field tests. b The velocity of C57BL/6 mice in open field tests.
The full blots of PSD95, synaptophysin and NeuN expression in hippocampus and prefrontal cortex of mice infected with A. cantonensis detected by Western blotting. a b The level of PSD95, synaptophysin and NeuN expression in hippocampus and prefrontal cortex of mice infected with A. cantonensis.
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Jhan, KY., Chang, PK., Cheng, CJ. et al. Synaptic loss and progression in mice infected with Angiostrongylus cantonensis in the early stage. J Neuroinflammation 19, 85 (2022). https://doi.org/10.1186/s12974-022-02436-8