Saturated very long-chain fatty acids (VLCFA, ≥ C22), enriched in brain myelin and innate immune cells, accumulate in X-linked adrenoleukodystrophy (X-ALD) due to inherited dysfunction of the peroxisomal VLCFA transporter ABCD1. In its severest form, X-ALD causes cerebral myelin destruction with infiltration of pro-inflammatory skewed monocytes/macrophages. How VLCFA levels relate to macrophage activation is unclear. Here, whole transcriptome sequencing of X-ALD macrophages indicated that VLCFAs prime human macrophage membranes for inflammation and increased expression of factors involved in chemotaxis and invasion. When added externally to mimic lipid release in demyelinating X-ALD lesions, VLCFAs did not activate toll-like receptors in primary macrophages. In contrast, VLCFAs provoked pro-inflammatory responses through scavenger receptor CD36-mediated uptake, cumulating in JNK signalling and expression of matrix-degrading enzymes and chemokine release. Following pro-inflammatory LPS activation, VLCFA levels increased also in healthy macrophages. With the onset of the resolution, VLCFAs were rapidly cleared in control macrophages by increased peroxisomal VLCFA degradation through liver-X-receptor mediated upregulation of ABCD1. ABCD1 deficiency impaired VLCFA homeostasis and prolonged pro-inflammatory gene expression upon LPS treatment. Our study uncovers a pivotal role for ABCD1, a protein linked to neuroinflammation, and associated peroxisomal VLCFA degradation in regulating macrophage plasticity.
Tight regulation of lipid metabolism is essential for the innate immune response. This is especially evident in macrophages, for which reprogramming the lipid composition in response to different activation signals is key for their opposing roles in pro- and anti-inflammatory processes . The metabolic program that supports pro-inflammatory macrophage reactions relies on both, glycolysis to cover the rapid energy demand and lipogenesis to produce pro-inflammatory mediators and saturated fatty acids for membrane reconfiguration [2, 3].
Recently, an unexpected role for extracellular saturated very long-chain fatty acids (VLCFAs, ≥ C22) in modulating pro-inflammatory signalling in human monocytes as well as murine bone marrow-derived macrophages was identified . In their study, Kanoh et al. demonstrated that serum-derived GM3 gangliosides enriched with saturated VLCFAs enhanced LPS-induced signalling of the pattern recognition receptor toll-like receptor 4 (TLR4), whereas gangliosides containing saturated long-chain fatty acids (LCFAs) such as C16:0 had an antagonistic effect . Synthesis of saturated VLCFAs is accomplished by a family of ER-embedded substrate-specific enzymes called elongation of very long-chain fatty acids (ELOVL) proteins 1–7 that catalyse the first, rate-limiting step within the LCFA and VLCFA elongation cycle. Degradation of VLCFAs is localized in peroxisomes, where the fatty acids are broken down by β-oxidation. Under physiological conditions, VLCFAs are of low abundance but they accumulate strongly in tissues and body fluids of patients affected by the inherited neurodegenerative disorder X-linked adrenoleukodystrophy (X-ALD, OMIM #300100). In X-ALD, mutations in the ATP-bindingcassettesubfamilyDmember1 (ABCD1) gene inactivates the function of the encoded VLCFA transporter, resulting in impaired degradation of VLCFAs within peroxisomes [5, 6]. In the severest neuroinflammatory presentation (cerebral X-ALD, CALD), patients suffer from rapidly progressive myelin destruction and axonal damage with infiltration of peripheral immune cells such as monocytes and T cells [5, 7]. If the inflammatory demyelination in CALD is detected at an early stage, the degradation of myelin and axons can be stopped by haematopoietic stem cell (HSC) transplantation or gene therapy [8,9,10]. Of note, the HSC-derived immune cell lineage most affected by VLCFA accumulation in X-ALD are monocytes/macrophages . Thus, these cells are not only central in the onset and progression of CALD but, upon correction by HSC-transplantation, can also stop the neuroinflammation in affected patients. Consistent with this concept, we previously demonstrated that X-ALD macrophages are pro-inflammatory skewed and that their plasticity to adapt an anti-inflammatory phenotype is impaired . Exactly how VLCFA levels contribute to the pro-inflammatory activation state of macrophages is unclear.
In this study, we specifically investigated the role of saturated VLCFAs in macrophage activation. We found that excessive intracellular VLCFAs prime the macrophage membrane for pro-inflammatory responses and, when added extrinsically to macrophages, activate the c-Jun N-terminal kinase (JNK) pathway cumulating in the release of pro-inflammatory chemokines. Following an acute pro-inflammatory response, normal macrophages rapidly cleared saturated VLCFAs by increased peroxisomal β-oxidation involving LXR-mediated upregulation of the VLCFA transporter ABCD1. Consequently, in X-ALD macrophages, the ABCD1 deficiency and impaired ability to degrade saturated VLCFAs prolonged the pro-inflammatory gene expression pattern. Accordingly, our data uncover a pivotal role for ABCD1 and the associated peroxisomal β-oxidation of VLCFAs in resolving the inflammatory state of macrophages.
Materials and methods
X-ALD patients and healthy volunteers
The study included peripheral blood samples from 11 adult X-ALD patients (age 23–45 years, mean = 36 years) and 15 healthy volunteers (age 24–61 years, mean = 40 years). X-ALD patients displayed clinical symptoms of axonopathy in the spinal cord but no signs of cerebral involvement (CALD) at MRI. The study was approved by the Ethical Committee of the Medical University of Vienna (EK1462/2014) and informed consent was obtained from participating X-ALD patients and healthy volunteers. In addition, leukoreduction system chambers derived from 46 healthy donors (median age = 33) were purchased from the General Hospital of Vienna, Austria, and used for monocyte isolation.
Isolation of human monocytes from peripheral blood
Primary CD14+ monocytes were isolated from the peripheral blood by Ficoll density-gradient centrifugation (PAN-Biotech) and positive selection for CD14+ cells using MACS microbeads and the LS column system (Miltenyi Biotec) according to the manufacturer’s instructions.
Differentiation and activation of human macrophages
CD14+ monocytes were differentiated in RPMI medium (Sigma Aldrich) containing 1% penicillin/streptomycin, 1% glutamine, 1% Fungizone (all Invitrogen) and 10% FCS (Gibco Life Technologies), supplemented with 50 ng/ml human recombinant M-CSF (PeproTech) for 7 days. After differentiation, either the LXR agonist T0901317 (Calbiochem), the LXR antagonist GSK1440233 (GlaxoSmithKline, ) or the natural LXR ligand 25-hydroxycholesterol (25-HC, Merck) was added for up to 24 h. For time response analysis, M-CSF-differentiated macrophages were stimulated with 100 ng/ml LPS (Escherichiacoli 055:B5, Cat.no. L4005, Sigma) or 100 µM C26:0 (Merck, dissolved in EtOH) as indicated. C16:0 (Merck) was dissolved in EtOH and used at a final concentration of 100 µM.
Jurkat and THP-1 reporter assays
Jurkat reporter cells expressing E6-1-NFκB::eGFP-TLR2/1, E6-1-NFκB::eGFP-TLR2/6 or E6-1-NFκB::eGFP-TLR4/CD14, as well as reporter THP-1 E6-1-NFκB::eGFP-TLR4/CD14 cells were previously described [14, 15]. Cells (5 × 10/well) were stimulated with TLR ligands flagellin, LPS, PAM3CSK4 or MALP2 (all purchased from Invivogen, San Diego, CA and used at a final concentration of 100 ng/ml) or saturated LCFA (C16:0), saturated VLCFAs (C24:0, C26:0) or mono-unsaturated LCFA (C18:1) in 96 well plates. After 24 h, cells were harvested and NFκB-eGFP expression was assessed via flow cytometry using FACS Calibur with CellQuest software (both BD Biosciences, San Jose, CA). Data were analysed using FlowJo software version 10.6.1 (Tree Star, Ashland, OR).
RNA isolation and RT-qPCR
RNA was isolated from differentiated macrophages using the RNeasy Mini Kit (Qiagen) according to the manufacturer’s instructions. cDNA was synthesized from total RNA samples using the iScript™ cDNA synthesis Kit (Bio-Rad). qPCR was performed with the CFX96™ Real-Time PCR Detection System (Bio-Rad) for each cDNA sample in technical duplicates. Relative mRNA levels were detected by SYBRGreen incorporation and calculated by the 2−∆∆Cq method using HPRT1 or RACK1 for normalization and a control sample on each plate as internal reference. To determine the absolute mRNA abundance of ABCD1, and for normalization, HPRT1, quantification was carried out using standard curves of known copy numbers of linearized plasmids containing ABCD1 and HPRT1 cDNA. Sequences of primers are listed in Additional file 1: Table S1.
RNA-seq and bioinformatic analysis of the data
First, the quality of RNA was assessed by using the Agilent 2100 Bioanalyzer (Agilent Technologies). All used samples had RNA integrity number (RIN) values higher than 8.6. Next, mRNA was selected using poly-A enrichment, followed by the cDNA library construction. The sequencing was performed at the Biomedical Sequencing Facility of the Medical University of Vienna, Austria, on Illumina HiSeq2000 using single end 50 bp reads and 10 samples per lane. The initial mapping of reads to the hg38 human genome assembly was done using STAR . Count tables were produced and further analysis was performed with R 4.0.0 using DESeq2 . The likelihood ratio test was performed to test the effect of the group, with the reduced model containing the isolation time only. The comparison (cells from X-ALD patients to cells from healthy controls) was performed with alpha set to 0.01 using the Benjamini–Hochberg method for adjusting p-values. The data were corrected for gene length and then tested using the Wallenius approximation. The resulting p-values were corrected using the Benjamini–Hochberg method. The results from RNAseq analysis were further interpreted using the following workflows: (1) GO Biological Processes (GO:BP) annotation enrichment analysis was performed using Enrichr (https://maayanlab.cloud/Enrichr/) as previously described [18, 19]; (2) biological network analysis and visualization was performed in NetworkAnalyst 3.0 (https://www.networkanalyst.ca/) and inbuilt generic PPI database (IMEx Interactome), with a minimal network being used as described ; and (3) volcano plot of differentially expressed genes belonging to GO:BP was prepared using R 4.0.0 with ggplot2, ggrepel and ggsci.
Western blot analysis
Differentiated macrophages were lysed in RIPA buffer containing protease inhibitors (Roche cOmplete), mixed with 5× sample buffer before separation of proteins on a denaturing 7.5% polyacrylamide gel by discontinuous electrophoresis (SDS–PAGE), followed by semidry blotting onto nitrocellulose membrane. To determine the phosphorylation states of proteins, samples were treated with a cocktail of protease and phosphatase inhibitors (1× Roche cOmplete protease inhibitors, 1× PhosStop (Sigma), 1 mM Na3VO4, 1 mM NaF, 1 mM PMSF). After the blot-transfer, equal protein load was monitored by Ponceau staining. All blots were blocked with 4% non-fat dry milk powder (w/vol) in TBS-T and probed with primary antibodies against the human ABCD1 protein (Euromedex ALD-1D6-AS, clone 2AL-1D6, 1:10,000), rabbit anti-human SAPK/JNK (Cell Signaling, #9252; 1:1000), rabbit anti-human Phospho-SAPK/JNK (Thr183/Tyr185; Cell Signaling, #9251; 1:1000), rabbit anti-human NF-κB p65 (Cell Signaling Technology, #4764, 1:1000), rabbit anti-human phospho-NF-κB p65 (Ser536; Cell Signaling Technology, #3033, 1:1000) and mouse anti-human β-actin (Chemicon, 1:100,000) followed by goat anti-mouse or anti-rabbit secondary antibodies conjugated with horseradish peroxidase (Dako, 1:30,000). Blots probed with anti-phosphorylated NF-κB p65 and anti-phosphorylated JNK antibody, were stripped with acidic stripping buffer (0.2 M glycine, 0.5 M NaCl, pH 2.5) three times for 5 min and washed thoroughly with TBS-T before re-probing with anti-NF-κB p65 or anti-JNK to enable normalization to protein levels. For detection, the Immobilon Western HRP Substrate Peroxide Solution and Immobilon Reagent (Millipore) were used with the ChemiDoc Imaging System and Image Lab software (Bio-Rad).
The chemokines CXCL8, CCL3, CCL4, CCL11, CX3CL1, CXCL1, CCL7, IL-12p40, CCL22, IL-15, CXCL10 and CCL2 were measured in the supernatant collected from cultured macrophages using Luminex® bead array technology (HCYTOMAG-60K-06.Hum, Merck) according to the manufacturer’s instruction.
Fatty acid analysis
The total amounts of saturated, mono-unsaturated and polyunsaturated fatty acids were determined by electrospray ionization mass spectrometry (ESI–MS) as described by Valianpour et al.  and normalized to macrophage protein content.
β-Oxidation of 14C-labelled C16:0 and C26:0
Radiolabelled fatty acids, [1-14C]-palmitic acid (C16:0; ARC 0172A) and [1-14C]-hexacosanoic acid (C26:0; ARC 1253), were obtained from American Radiolabeled Chemicals (St. Louis, MO, USA). Free fatty acids in ethanol were aliquoted into glass reaction tubes, dried under a stream of nitrogen and solubilized in 10 mg/ml α-cyclodextrin by ultrasonication. The reaction mix contained 4 µM of labelled fatty acids, 2 mg/ml α-cyclodextrin, 30 mM KCl, 8.5 mM ATP, 8.5 mM MgCl2, 1 mM NAD+, 0.17 mM FAD, 2.5 mM l-carnitine, 0.16 mM CoA, 0.5 mM malate, 0.2 mM EDTA, 1 mM DTT, 250 mM sucrose and 20 mM Tris–Cl, pH 8.0. Reactions were started by addition of 5 × 106–2 × 107 cells, carried out for 1 h at 37 °C and stopped by addition of KOH and heating to 60 °C for 1 h. After protein precipitation by HClO4, a Folch partition was carried out, and 14C-acetate was determined in the aqueous phase by scintillation counting using the Perkin Elmer Tri-Carb 4910TR Scintillation Counter.
Flow cytometry analysis
For detachment, adherent macrophages were washed with PBS, incubated with 300 µl 10× Gibco™TrypLE™Select (Gibco, Life Technologies) at 37 °C for 15 min and gently collected with a cell scraper after adding 300 µl PBS. Before staining, Fcγ receptor blockage was performed by incubating the cells with 3 mg/ml Beriglobin P (#I4506, Sigma Aldrich) at 4 °C in the dark for 30 min. To determine the purity of isolated primary CD14+ monocytes, cells were stained with either FITC-conjugated mouse anti-human CD14+ REAfinity (#130-110-518, Miltenyi Biotec), FITC-conjugated human IgG1 REAfinity control (#130-113-437, Miltenyi Biotec), PE-conjugated mouse anti-human CD19 monoclonal (clone LT19, #130-091-247, Miltenyi Biotec), PE-conjugated monoclonal isotype control (#120-002-723, Miltenyi Biotec), FITC-conjugated mouse anti-human CD3 monoclonal (#130-080-401, Miltenyi Biotec) and FITC-conjugated mouse IgG2a isotype control (#130-091-837, Miltenyi Biotec) antibodies. To determine the frequency of CD86+ macrophages, cells were stained using anti-CD86-PerCP-eFluor 710 (eBioscience) or isotype control (IgG2b-PerCP-eFluor-710, eBioscience) antibodies. Data were acquired using a BD LSRFortessa™ flow cytometer and analysed using FlowJo software, Treestar Inc.
Calcein Red-AM viability assay
The viability of human primary macrophages exposed to VLCFAs was investigated using Calcein Red-AM staining. Cells were incubated with C26:0 (10, 20, 50 or 100 µM with final concentration of EtOH filled up to 1%) for 24 h, washed with PBS and stained for 30 min with 1 µM Calcein Red-AM (Biolegend). Plates were imaged using the IncuCyte SX5 live-cell analysis system (Sartorious) with a 10× objective for phase contrast and the orange channel (acquisition time 400 ms). Images were examined using the IncuCyte software. Phase images were subjected to segmentation adjustment of + 1. The analysis definition was optimized for the red channel, so that each red object corresponded to a viable cell by performing a Top-Hat segmentation with a threshold of 15 OCU, a radius of 10 µm, and an edge sensitivity of − 25. By using these settings, red object areas smaller than 80 µm2 were filtered out. Data were represented as the number of viable cells per image against phase area per image.
Macrophages were in vitro differentiated for 7 days on poly-l lysine coated coverslips before addition of C26:0 (100 µM) or the solvent ethanol. After 24 h of treatment, cells were washed with PBS, fixed in 3% paraformaldehyde for 20 min and again washed with PBS. For immunofluorescence, cells were permeabilized with 0.1% Triton for 5 min and blocked with 2% FCS, 2% BSA and 0.2% fish gelatin for 30 min, followed by PBS washing. For vinculin staining, cells were incubated for 2 h with a monoclonal mouse anti-human vinculin antibody (1:250, #V9264, Sigma). Filamentous (F)-actin was stained for 1 h using AlexaFluor488 Phalloidin (1:2000, ThermoFisher). Then, a secondary donkey anti-mouse IgG Cy3 antibody (1:400, #IR715-165-150, Jackson) was added for 1 h. After staining, cells were washed with PBS, nuclei stained with DAPI (1:2000) for 30 min in the dark before mounting in Mowiol. Fluorescence microscopy was carried out using an Olympus Ix71 inverted fluorescence phase contrast microscope with quantification done for 10 areas per coverslip containing approximately 130 cells. Macrophages displaying more than five podosomes were counted as podosome positive. For visualization of podosomes by confocal microscopy, a laser scanning LSM 700 (Zeiss) was used.
For statistical analysis, one-way ANOVA, two-tailed unpaired or paired Student’s t-test, ratio paired Student’s t-test as well as Mann–Whitney test were used as indicated in the figure legends. Multiple testing was corrected using Bonferroni adjustment. For post hoc analysis of time responses Fisher’s LSD multiple comparison test was used. P-values below 0.05 were regarded to indicate statistical significance. Graphs were produced and statistical results calculated using GraphPad Prism 8. Boxplots indicate median ± interquartile range, while whiskers show minimum and maximum. Bar graphs show individual data points with means ± standard deviations.
The raw data and count tables used in RNA-seq analysis are available through NCBI’s GEO repository under accession GSE217140. The R-markdown file used for the analysis of RNA-seq data presented in this paper is available in the repository: https://github.com/JureFabjan/macrophage_plasticity.
Intrinsically elevated VLCFAs due to ABCD1 deficiency prime the macrophage cell membrane for pro-inflammatory response
In X-ALD, the impaired ability of macrophages to degrade saturated VLCFAs results in exaggerated pro-inflammatory gene expression upon LPS stimulation . To uncover the mechanism by which saturated VLCFAs promote the pro-inflammatory response, we first applied whole transcriptome sequencing to assess how excessive VLCFA levels modulate the activation state of X-ALD macrophages. To avoid strong extrinsic pro-inflammatory cues associated with the onset of CALD that might cover subtle changes associated with intrinsic VLCFA accumulation in X-ALD cells, we included only X-ALD patients who had not developed cerebral involvement at the time of blood donation for this study.
Therefore, we isolated CD14+ monocytes from peripheral blood of nine adult male X-ALD patients lacking signs of brain inflammation and nine age and sex-matched healthy controls. The purity of the cells was determined by flow cytometry (Additional file 1: Fig. S1). The isolated monocytes were differentiated in vitro to homeostatic macrophages in the presence of M-CSF for 7 days before RNA-sequencing was performed. Pathway enrichment analysis revealed the most significant differences between X-ALD and control macrophages for the mRNA levels of genes related to the inflammatory response (Table 1).
Within this Gene Ontology (GO) category, the top upregulated genes were enriched for membrane receptors and membrane-associated proteins involved in pro-inflammatory signalling, such as C–Cchemokinereceptortype2 (CCR2) and F2R (proteaseactivatedreceptor1, PAR1), encoding two G-protein coupled receptors, and thrombospondin1 (THBS1). CCR2 mediates the transmigration of immune cells across brain endothelial cells during neuroinflammation [22,23,24,25,26], while F2R primes macrophages for LPS and IFNγ responses . THBS1 is a protein with anti-angiogenic properties, which is induced by saturated fatty acids and interacts with the fatty acid translocase CD36 to stimulate TLR4 signalling and monocyte/macrophage adhesion to the blood–brain barrier (BBB) [28, 29] (Fig. 1, Additional file 1: Fig. S2).
We also observed the induction of genes encoding secreted pro-inflammatory mediators, mainly chemokines, such as CXCL7 (also named pro-plateletbasicprotein, PPBP), CLL7, CXCL5 and CXCL8. CXCL7/PPBP is a fatty acid-induced chemokine elevated in atherosclerosis; CCL7 is a macrophage foam cell marker  recruiting monocytes to sites of inflammation and has a high affinity to CCR2. CXCL5 is a chemokine regulating macrophage cholesterol efflux as well as tissue remodelling/invasion. CXCL8 is a chemotactic factor for both neutrophils and macrophages that modulates cell adherence and formation of membrane protrusions but also acts on BBB permeability for immune cell infiltration [31, 32]. Furthermore, X-ALD macrophages had elevated expression of the pro-inflammatory cytokines tumournecrosisfactoralpha (TNF) and interleukin1beta (IL1B) and of the transcription factor c-FOS. As a heterodimer with c-JUN, cFOS constitutes AP-1, which is a transcription factor activated by the c-Jun N-terminal kinase (JNK) pathway linked to stress signals and migration. In contrast to the upregulated mRNAs encoding proteins involved in pro-inflammatory reactions, we found downregulated expression of factors attenuating the pro-inflammatory response of macrophages including: Interleukin36receptorantagonist (IL36RN), Interleukin36beta (IL36B), Alpha-1-acidglycoprotein1 (ORM1), Chitinase3-like1 (CHI3L1) and PhospholipaseA2GroupIID (PLA2G2D, Fig. 1, Additional file 1: Fig. S2).
When we visualized the functional interaction of the differentially expressed genes within the GO Term Inflammatory Response using the NetworkAnalyst software, we observed that the top three interaction nodes were CXCL8, TNF and FOS (Fig. 1B). Together, our results from whole transcriptome RNA-sequencing of X-ALD macrophages versus controls indicate that saturated VLCFAs set up the macrophage plasma membrane for pro-inflammatory activity, while simultaneously repressing factors regulating the balance between pro- and anti-inflammatory macrophage responses.
Saturated VLCFAs do not directly activate TLR signalling but propagate the pro-inflammatory macrophage response by stimulating the CD36–JNK axis
Saturated VLCFAs are particularly enriched in the brain, where these fatty acids function in decreasing myelin fluidity and providing a permeability barrier to insulate axons . With onset of neuroinflammation and associated myelin degradation, VLCFA-enriched lipids strongly accumulate in CALD brain lesions, where they constitute up to 67% of fatty acids in cholesterol esters . Thus, we next assessed how extracellular saturated VLCFAs act on macrophage activation. We first investigated whether C24:0 and C26:0, the VLCFAs most prominently accumulating in X-ALD, are recognized by the toll-like receptors TLR4, TLR2/1 or TLR2/6, which are endogenously expressed on the surface of macrophages. To test this hypothesis, we made use of modified Jurkat T cells, which endogenously express solely TLR5 but were engineered to overexpress either TLR4 homo- or TLR2/1 or TLR2/6 heterodimers together with an NFκB-eGFP reporter plasmid for downstream detection of NFκB signalling . In this assay, only the recognition of a matching ligand by the TLRs induces dimerization and internalization, leading to an NFκB-mediated downstream fluorescent signal that can be detected and quantified by flow cytometry. Using this reporter system, we found that exposure to different concentrations of C24:0 and C26:0 did not initiate a NFκB response (Fig. 2A), whereas cognate binding partners for each TLR receptor (flagellin, LPS, PAM3CSK4 and MALP2) induced NFκB signalling (Fig. 2A).
In addition, neither treatment with the saturated LCFA C16:0 nor with the mono-unsaturated LCFA C18:1 activated TLR signalling, thus confirming previous investigations . To further evaluate these findings in myeloid cells naturally equipped with TLR4, TLR2/1 and TLR2/6 receptors, we treated a monocytic THP-1 cell line stably expressing NFκB-eGFP  with either the fatty acids or the cognate ligands. However, also in these cells, neither the saturated VLCFAs C24:0 and C26:0 nor the LCFAs C16:0 and C18:1 were able to activate the NFκB pathway, despite clear positivity resulting from flagellin, LPS, PAM3CSK4 or MALP2 treatment (Fig. 2A). We finally confirmed our findings in primary human macrophages by Western blot analysis after exposure to C26:0, C16:0 or LPS for up to 24 h. In contrast to LPS, neither C26:0 nor C16:0 fatty acids induced activation of the NFκB pathway, as evidenced by absent phosphorylation of NFκB-p65 after treatment (Fig. 2B–D). The viability of primary human macrophages was not severely affected by C26:0 treatment (Additional file 1: Fig. S3). Collectively, these data show that the saturated VLCFA C26:0 and the saturated LCFA C16:0 are unable to elicit TLR4, TLR2/1 and TLR2/6 signalling in Jurkat T cells, monocytic THP-1 cells and primary monocyte-derived macrophages.
Besides NFkB as a master coordinator of pro-inflammatory macrophage activation, also stimulation of the c-Jun N-terminal kinase (JNK) pathway is key in the induction of inflammatory responses in macrophages including cell migration and cytokine production . Of note, saturated LCFAs but not polyunsaturated fatty acids (PUFAs) were previously identified as relevant JNK activators that induce clustering of the tyrosine kinase c-SRC in membrane subdomains, thereby leading to activation of JNK signalling [3, 37]. Therefore, we next assessed whether C26:0 elicits a pro-inflammatory response in macrophages by activating the JNK pathway. Indeed, Western blot analysis of human macrophages treated with C26:0 for up to 48 h revealed significantly increased JNK phosphorylation (JNK-P) levels that peaked at 3 h and persisted up to 6 h of incubation (Fig. 2F). As expected, both C16:0 and LPS induced JNK phosphorylation (Fig. 2E, G).
Due to their chemical properties, VLCFAs are unable to freely diffuse across the plasma membrane. Thus, we next asked whether the translocation through a protein-based mechanism could be a prerequisite for C26:0-mediated JNK signalling. Previously, the scavenger receptor CD36 was identified to be required for the uptake of saturated VLCFAs in COS-7 cells and absorption of dietary VLCFAs in the intestine of mice . To examine whether CD36 is involved in C26:0 inducing the JNK-pathway in macrophages, we stimulated the cells with C26:0 in the presence of the CD36 competitive inhibitor sulfosuccinimidyl oleate (SSO) . Indeed, inhibition of CD36 by SSO co-treatment significantly reduced activation of the JNK pathway in C26:0-exposed macrophages (Fig. 2H, Additional file 1: Fig. S4). Collectively, these data indicate that C26:0 needs to be transported into macrophages through the fatty acid translocase CD36 to promote intracellular JNK signalling.
Saturated VLCFA treatment specifically stimulates macrophages for chemokine secretion and upregulation of extracellular matrix-degrading enzymes
Having shown that CD36-mediated uptake of C26:0 stimulates the JNK pathway, we next assessed how this activation impacts pro-inflammatory responses in healthy human macrophages. Using FACS analysis, we found that incubation of macrophages with C26:0 increased the expression of CD86 (Fig. 3A), a surface activation marker previously identified to be upregulated on macrophages/microglial cells in active brain lesions of CALD patients .
In contrast, no significant modulation of the expression of classical pro-inflammatory cytokine genes such as IL6, TNF or IL1B was observed (Additional file 1: Fig. S5). To test whether VLCFA treatment promotes chemokine production, as suggested by the whole transcriptome analysis of X-ALD macrophages (Fig. 1), we used RT-qPCR to analyse the expression of CXCL8, CCL3 and CCL4 in macrophages treated with C26:0 for up to 24 h. We found that C26:0 significantly stimulated the expression of all three investigated chemokines (Fig. 3B). To further confirm the production of these chemokines at the protein level, we collected the supernatants of control macrophages incubated in the presence of C26:0 for 24 h and performed multiplex Luminex bead-based immunoassay for CXCL8 (IL-8), CCL3 (MIP-1α) and CCL4 (MIP-1β) and additionally for CCL11 (eotaxin), CX3CL1 (fraktaline), CXCL1 (GRO), CCL7 (MCP-3), IL-12p40, CCL22 (MDC), IL-15, CXCL10 (IP-10) and CCL2 (MCP-1) (Fig. 3C, Additional file 1: Fig. S6). We found a striking increase for the release of CXCL8 and CCL3 and a tendency towards elevated secretion of CCL4 upon C26:0 treatment (Fig. 3C). In contrast, VLCFA supplementation reduced the levels of CCL22 (Fig. 3C), CXCL10 and CCL7, whereas CCL2 and CXCL1 secretion was not affected (Additional file 1: Fig. S6). In this assay, we were unable to detect CCL11, CX3CL1, IL-12p40 and IL-15 in the macrophage supernatants both before and after VLCFA treatment. As C16:0 treatment was previously shown to impact CXCL8 expression , we next compared the effects of C26:0 and C16:0 on CXCL8, CCL3 and CCL4 gene expression. We found that incubation with C26:0 for 24 h resulted in significantly higher CCL3 mRNA levels and a trend towards increased CXCL8 and CCL4 expression when compared to C16:0 (Fig. 3D). We also assessed whether the production of chemokines in response to VLCFAs involves the CD36 fatty acid translocase by co-incubating macrophages with C26:0 and the CD36-inhibitor SSO. Our data revealed that inhibiting CD36 activity significantly interfered with C26:0 mediated upregulation of CXCL8 mRNA levels, indicating that CXCL8 chemokine production in response to VLCFAs is CD36-dependent (Fig. 3E).
In X-ALD macrophages, the lack of ABCD1 with associated VLCFA accumulation is not only linked to chemokine upregulation, but also to increased expression of genes associated with BBB adhesion and invasion (Fig. 1). Therefore, we also tested whether treatment of healthy control macrophages with either C26:0 or C16:0 for 24 h alters the expression of genes responsible for the proteolytic degradation of extracellular matrix molecules at the basal lamina of the BBB. The mRNA levels analysed included genes encoding matrix metalloproteinases (MMP9 and MMP14) as well as the urokinase plasminogen activator surface receptor (PLAUR), which induces extracellular matrix degradation through protease activity. We found that C26:0 significantly induced the expression of MMP9 and PLAUR and tendentially (p = 0.053) increased MMP14 mRNA levels after 24 h of treatment (Fig. 3F). Concomitant with these results, we observed that C26:0 treatment increased the frequency of macrophages presenting podosomes (Fig. 3G, Additional file 1: Fig. S7), which are dynamic adhesive structures involved in extracellular matrix degradation through recruitment of matrix lytic enzymes such as metalloproteinases.
Macrophages remodel their VLCFA content in response to inflammation
Treatment of macrophages with the saturated VLCFA C26:0 promotes JNK signalling and cumulates in the release of pro-inflammatory mediators, mainly chemokines. In addition, our RNA-seq data suggest that VLCFA accumulation in X-ALD macrophages predisposes them for pro-inflammatory responses. Based on these observations we hypothesized that macrophages need to tightly regulate their intracellular VLCFA levels to prevent exaggerated inflammatory responses. To test this notion, we analysed how the cellular VLCFA content changes with the onset and duration of pro-inflammatory stimulation. We applied electrospray ionization mass spectrometry (ESI–MS) to determine the fatty acid profile after hydrolysis of fatty acyl esters, which provides a good estimation of membrane lipid abundance of macrophages during different stages of pro-inflammatory activation. We found that upon LPS treatment of healthy control macrophages, saturated and mono-unsaturated VLCFAs (C24:0 and C26:0 as well as C24:1 and C26:1) increased during the immediate/early pro-inflammatory response, when acute mediators like TNFα are produced (up to 3 h after LPS addition, Fig. 4A, B).
The amplification of VLCFA levels was paralleled by a general increase in saturated and mono-unsaturated LCFAs as well as PUFAs, thus confirming previous reports . The elevation in macrophage fatty acid content was reflected in the upregulation of enzymes involved in de novo biosynthesis of saturated LCFAs (fattyacidsynthetase, FASN) and PUFAs (fattyaciddesaturase2, FADS2 and elongationofverylong-chainfattyacids7, ELOVL7) at 3 h post-LPS application (Fig. 4C). The levels of stearoyl-CoAdesaturase (SCD1, aliasFADS5), responsible for the conversion of saturated LCFAs to mono-unsaturated LCFAs, remained unchanged in the early inflammatory state (Fig. 4C). With the onset of resolution of the pro-inflammatory state (pro-resolution) and decreasing expression of the acute mediator TNF and peak expression of the late response gene IL12B (12 h post-LPS addition, Fig. 4B), our data revealed a selective reduction of saturated and mono-unsaturated VLCFAs. This was accompanied by significant downregulation of ELOVL1, encoding the enzyme involved in saturated VLCFA synthesis  (Fig. 4A, C). In contrast, saturated and mono-unsaturated fatty acids with shorter chain lengths (≤ C22) or highly unsaturated VLCFAs (C24:6 and C24:5) still remained elevated 24 h post-LPS application, despite preceding downregulation of enzymes involved in their synthesis (FASN, FADS2 and SCD1, Fig. 4C). The expression of ELOVL7, which is required for synthesis of anti-inflammatory PUFAs, remained upregulated during the resolution phase (Fig. 4C).
In X-ALD macrophages, VLCFA accumulation combined with an impaired ability to remodel their elevated VLCFA content prolonged the expression of acute pro-inflammatory mediator genes when compared to healthy control cells at 24 h post-LPS application (Fig. 4D), thus confirming our previous results [42,43,44]. Of note, the increased pro-inflammatory gene expression in X-ALD macrophages upon LPS-treatment was also reflected by a trend towards elevated ELOVL7 expression, an enzyme strongly upregulated with LPS-treatment (Fig. 4C), when compared to control cells (Fig. 4D). Regarding FADS2 and SCD1, X-ALD cells were able to downregulate the expression of these genes to similar extents as controls (Fig. 4D). Together, our results demonstrate that macrophages quickly metabolize saturated and mono-unsaturated VLCFAs with transition from the pro-inflammatory phase to the onset and establishment of resolution. In ABCD1-deficient (X-ALD) macrophages with impaired catabolism of VLCFAs, the excess of VLCFAs impacts the plasticity of macrophages by prolonging the pro-inflammatory response.
The rate of peroxisomal VLCFA degradation is linked to the pro-inflammatory status of macrophages
Our results suggest that macrophages must clear VLCFAs to efficiently terminate the pro-inflammatory response. As VLCFAs are degraded by β-oxidation within peroxisomes, we next asked how the peroxisomal β-oxidation activity of macrophages responds to LPS treatment. Therefore, we analysed the VLCFA degradation rate by peroxisomes and, for comparison, the rate of breakdown of LCFAs by mitochondrial β-oxidation in LPS-stimulated primary human macrophages at the onset and up to 24 h post-application of the pro-inflammatory stimulus. The degradation of VLCFAs requires the activation of the fatty acids through addition of coenzyme A and transport into the organelles. In the peroxisomal matrix, the β-oxidation, consisting of dehydrogenation, hydration, oxidation and thiolytic cleavage, occurs resulting in the shortening of the acyl-CoA chain by two carbons per cycle (Fig. 5B).
In the in vitro β-oxidation assay, this release of water-soluble C2 units from radioactively labelled C26:0 or C16:0 is measured. Accompanying the general increase in fatty acid content early upon LPS-stimulation (Fig. 4A), we observed tendentially lowered rates for both peroxisomal and mitochondrial β-oxidation rates accompanying pro-inflammatory activation in macrophages (Fig. 5A). However, with the onset of pro-inflammatory resolution, aimed to terminate the pro-inflammatory response (24 h post-addition of LPS), only the peroxisomal fatty acid β-oxidation was significantly elevated, thus returning to pre-treatment levels (Fig. 5A).
To elucidate whether the increased peroxisomal β-oxidation upon entering the pro-inflammatory resolution phase is caused by a concerted upregulation of peroxisomal genes involved in VLCFA degradation, we treated macrophages with LPS and harvested cells at 3, 12 and 24 h post-LPS application. We assessed expression of ABCD1, encoding the rate-limiting VLCFA importer, as well as of the peroxisomal β-oxidation enzymes, acyl-CoAoxidase1 (ACOX1) and hydroxysteroid17-betadehydrogenase4 (HSD17B4, encoding D-bifunctional protein, DBP) (Fig. 5B). We found that ABCD1 was significantly upregulated starting at 12 h post-application of LPS at both mRNA and protein level (Fig. 5C, D), thus being induced concurrently with peroxisomal β-oxidation activity at resolution. Intriguingly, our analysis revealed that within the acute pro-inflammatory response (3 h post-LPS addition), expression of both ACOX1 and HSD17B4 were significantly downregulated in macrophages (Fig. 5E). We also tested whether ABCD3, encoding another peroxisomal fatty acid transporter that imports long-chain unsaturated-, long branched-chain- and long-chain dicarboxylic fatty acids for peroxisomal degradation, is modulated by pro-inflammatory stimulation and thus, would contribute to the increased fatty acid levels in activated macrophages. Indeed, ABCD3 was significantly downregulated early upon LPS application and remained repressed at 24 h post-LPS addition, thus further lending support to this concept (Fig. 5E).
To assess how genes involved in LCFA/VLCFA metabolism and peroxisomal genes in general respond to pro-inflammatory activation of macrophages, we retrieved transcriptomics data from the Gene Expression Omnibus (GEO) dataset GSE85333 , obtained from macrophages with a similar differentiation and LPS activation protocol as used in our study. By reanalysing this data set, we observed prominent upregulation of ABCD1, confirming our own results, and of a subset of genes including long-chain acyl-CoA synthetase (ACSL) family members (ACSL1, ACSL4 and ACSL5) involved in CoA-activation of fatty acids, which is a prerequisite for entering metabolic pathways like β-oxidation. Interestingly, most genes encoding peroxisomal proteins, including ABCD3, were repressed 7 and 24 h post-LPS addition (Fig. 5F), in line with our data. Collectively, these findings show that after the acute pro-inflammatory response to LPS, with the onset of resolution, macrophages specifically upregulate the VLCFA transporter ABCD1 to enable degradation of VLCFAs within peroxisomes. Despite this induction of ABCD1, the majority of peroxisomal genes including those involved in plasmalogen synthesis (alkylglycerone phosphate synthetase, AGPS; glyceronephosphate O-acyltransferase; GNPAT; fatty acyl-CoA reductase 2, FAR2), were found to be downregulated.
VLCFA degradation in macrophages during resolution of inflammation involves LXR-mediated upregulation of ABCD1 expression
As ABCD1 appears to be the molecular switch for quick and selective modification of VLCFA levels in LPS-activated macrophages, we investigated the underlying regulatory mechanism of ABCD1 upregulation at the transition to pro-inflammatory resolution. The nuclear oxysterol receptor and transcription factor liver X receptor (LXRα, also termed NR1H3), a crucial key player in regulating lipid metabolism including cholesterol export, promotes both pro-resolution and anti-inflammatory activity in macrophages [46, 47]. As the onset of efficient resolution of the pro-inflammatory state seems to necessitate clearance of VLCFAs, we hypothesized that ABCD1 expression could be modulated by LXR. Transcription of LXR itself is downregulated during the acute pro-inflammatory response and upregulated with the start of resolution , also in our paradigm (Fig. 6A).
The activation of LXR target genes is triggered by the binding of oxysterol ligands such as 25-hydroxycholesterol (25-HC), which is produced by the enzyme cholesterol-25-hydroxylase (CH25H). Thus, we first determined whether upregulation of ABCD1 occurs subsequent to 25-HC production by CH25H in our experimental conditions. Our analysis revealed a robust activation of CH25H expression at 3 h post-LPS application (Fig. 6B), thus preceding ABCD1 induction and confirming previous investigations . Addition of 25-HC, the product of CH25H, to the cell culture medium, resulted in significantly upregulated ABCD1 mRNA levels in 25-HC-stimulated human macrophages (Fig. 6C). To confirm that the 25-HC-mediated induction of ABCD1 involves LXR activity, we added the synthetic LXR antagonist GW1440233, which upon co-treatment abrogated 25-HC-induced ABCD1 expression (Fig. 6C). To investigate this interaction in more detail, we also applied the synthetic LXRα agonist T0901317 and, indeed, detected a significant upregulation of ABCD1 mRNA and protein levels by RT-qPCR and Western blot analysis, respectively (Fig. 6D, E). Consistent with our hypothesis that LXR mediates the upregulation of ABCD1 with the start of resolution, we observed that the LXR antagonist GW1440233 abolished induction of ABCD1 in LPS-treated macrophages (Fig. 6F). Together, our data demonstrate that LXR-mediated induction of ABCD1 expression enables peroxisomal VLCFA degradation in macrophages entering the pro-resolution state (Fig. 6G).
Changing the cellular lipid composition has been proposed as a prerequisite for macrophages to shape their immune response to environmental conditions . Here, we provide further evidence for this concept by demonstrating a reciprocal relationship between ABCD1-mediated peroxisomal degradation of saturated VLCFAs and the resolution of pro-inflammatory gene expression in macrophages. Thus, our findings directly link saturated VLCFA levels with a pro-inflammatory, pro-invasive phenotype (Fig. 7). Further, these results lend support to the idea that pharmacological modulation of these fatty acids in monocytes, macrophages and probably microglial cells could be beneficial towards altering the exaggerated innate immune response associated with neuroinflammation in X-ALD patients. Of note, the LPS-mediated upregulation of ABCD1, which is the rate-limiting factor in peroxisomal VLCFA degradation , was concurrent with repression of most peroxisomal genes including those involved in β-oxidation. This is the opposite pattern of that observed upon herpesvirus infection of B cells, where pathogen-induced downregulation of ABCD1 occurs despite general induction of peroxisomes . This would allow both accumulation of VLCFAs, which are needed by the virus, and exploitation of other peroxisomal functions required for viral replication . Accordingly, ABCD1 could represent a dynamic regulatory switch point in immune cells either promoting or preventing VLCFA accumulation to modulate pro-inflammatory responses associated with the defence against pathogens.
Saturated fatty acids are thought to create a receptive environment within the plasma membrane that enables the assembly of cholesterol-dependent pro-inflammatory signalling networks . Consistently, our whole-transcriptome analysis of X-ALD macrophages revealed that elevation of saturated VLCFAs primarily increased the expression of membrane receptors and membrane-associated proteins functioning in the pro-inflammatory response. In this regard, the membrane ordering property of saturated VLCFAs could promote sphingolipid- and cholesterol-rich lipid nanodomains and, thus, culminate in the formation of so called inflammarafts . By incorporating ion channels, membrane proteins and enzymes involved in pro-inflammatory signalling, these specialized clustered membrane structures serve as an organizing platform for active molecule complexes such as TLR dimers . Accordingly, inflammarafts are thought to enable downstream signal transduction in macrophages upon recognition of danger signals . A typical characteristic of inflammarafts is the increased cholesterol content per raft. In this respect, it is notable that next to the inflammatory response, also cholesterol and sterol biosynthesis were among the top dysregulated pathways in X-ALD macrophages. Aside from being involved in membrane organization, saturated fatty acids are also increasingly recognized for their roles in protein lipidation. Recently, it was shown that the reversible attachment of C16:0 (palmitoyl-group) to proteins including CD36 stimulates macrophage responses linked to intracellular signalling or chemotaxis . Importantly, next to palmitoylation, also saturated VLCFAs are used for protein acylation, as shown for processes associated with inflammatory programmed cell death, where limiting the amount of VLCFAs decreased membrane recruitment of proteins involved in necroptosis . Accordingly, the identity of the added acyl chain, such as C16:0 or C26:0 with different affinities for membranes and cholesterol-rich lipid plasma membrane nanodomains , could be central to the regulatory effects of lipidation of proteins and in fine tuning macrophage responses to specific danger cues.
In our study, we demonstrate that ABCD1-deficient X-ALD macrophages, highly accumulating VLCFA lipid species, have increased expression of genes linked to JNK signalling, such as c-FOS, interacting protein 2 (TRAF3IP2), TNF, IL1B and CXCL8 under steady-state conditions when compared to healthy controls. The extracellular exposure of healthy macrophages to free VLCFAs also triggered JNK signalling in a CD36-dependent manner, with robust expression and release of chemokines including CXCL8. Thus, contrary to the common theory proposing that saturated VLCFAs promote pro-inflammatory responses by stimulating TLR signalling, our data provide evidence refuting that C26:0, the saturated VLCFA accumulating most prominently in X-ALD patients, directly activates TLRs but rather stimulates pro-inflammatory macrophage response through the JNK pathway. Of note, the JNK pathway has been proposed as an important player also in other neurological disorders such as multiple sclerosis (MS). In MS, upregulation of JNK activity has been observed in peripheral blood mononuclear cells of relapsing patients when compared to healthy controls . In addition, increased JNK phosphorylation, indicative of JNK signalling, was found in the acute disease phase of experimental autoimmune encephalomyelitis rats, an animal model mimicking neuroinflammation in MS . Given the importance of the JNK pathway in pathological conditions, pan-JNK inhibition has been proposed as a novel treatment strategy for diseases associated with neurodegeneration and neuroinflammation [60, 61] and, thus, might also be of advantage in the context of X-ALD.
The rapidly progressive myelin destruction in patients with cerebral X-ALD is associated with a disturbed BBB and infiltration of peripheral monocytes/monocyte-derived macrophages and, to a lower extent, T cells . Therefore, important insight can be derived from our observations of stimulated chemokine expression in VLCFA-accumulating X-ALD macrophages and enhanced release of chemokines from healthy control macrophages exposed to C26:0. These findings indicate a specific role of saturated VLCFAs in stimulating chemotaxis and possibly BBB transmigration to sites of brain inflammation. Upon crossing the brain endothelium, monocytes differentiate to macrophages and accumulate in perivascular cuffs. To reach the inflamed sites of the brain parenchyma, macrophages release enzymes to remodel the extracellular matrix at the basal lamina of the BBB. Consistently, our data revealed not only increased expression of chemokines and chemokine receptors in X-ALD macrophages but also increased mRNA levels for genes encoding adhesion molecules modulating the interaction of peripheral immune cells with the BBB (THBS1; integrinsubunitalphaL, ITGAL) or proteins initiating the plasminogen cascade resulting in matrix turnover and cell invasion such as CXCL7. Further, increased VLCFA levels in X-ALD macrophages altered the expression of molecules associated with extracellular matrix remodelling (CXCL5, PLAUR and hyaluronidase1, HYAL1) and formation of membrane protrusions, as well as the modulation of BBB integrity for immune cell infiltration (CXCL8). Hence, our results pinpoint a mechanism by which VLCFA accumulation modulates X-ALD cells for invasive migratory behaviour. Our observation that macrophages respond to C26:0 treatment by increasing podosome formation lends further support for this concept. Of note, a recent report described reduced basal cell migration speed in a human neutrophil cell line with diminished VLCFA levels due to a knockdown of ELOVL1, the enzyme involved in VLCFA synthesis , thus further highlighting the role of VLCFAs in migratory behaviour. Whether indeed the high VLCFA levels in X-ALD macrophages amplify their ability to infiltrate the brain parenchyma upon onset of neuroinflammation remains to be clarified. Among the upregulated genes, CCR2 encodes the main chemokine receptor required for monocyte migration from the bone marrow to inflammatory sites and plays a key role in the damaging effects of neuroinflammation following traumatic brain injury [64, 65]. Increased levels of CCR2 and CCL7 are not only observed in X-ALD macrophages, but have also been found in inflammatory brain lesions of X-ALD patients . As classical anti-inflammatory treatment paradigms including application of IFNβ were unable to stop the neuroinflammation in X-ALD patients , pharmacological inhibition of factors involved in trans-endothelial migration across the BBB such as CCR2 or CXCL8 might be of interest in the context of developing alternative treatment strategies for the inflammatory cerebral form of X-ALD.
Taken together, we here show that the cellular content of saturated VLCFAs is tightly linked to the activation state of human macrophages and that ABCD1-mediated peroxisomal degradation of VLCFAs is pivotal for efficiently resolving the pro-inflammatory response. Thus, with saturated VLCFAs being linked to neuroinflammation and neurodegeneration, our findings on how these fatty acids modulate macrophage activation complements the current knowledge of lipid metabolism as a driving force in inflammation.
Availability of data and materials
The raw data and count tables used in RNA-seq analysis are available through NCBI's GEO repository under accession GSE217140. The R-markdown file used for the analysis of RNA-seq data presented in this paper is available in the repository: https://github.com/JureFabjan/macrophage_plasticity. All other data are included in the manuscript or in the Additional file 1 and are available from the corresponding author upon request.
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We would like to thank all the patients and healthy volunteers who participated in this study. In addition, we are grateful for excellent technical assistance by Martina Rothe. We also acknowledge the help of Prof. Andreas Spittler from the Flow Cytometry Core Facility and of Dr. Michael Schuster from the Biomedical Sequencing Facility of the Medical University of Vienna, Austria.
This work was supported by the Austrian Science Fund KLI 837-B to IW; DOC 33-B27 to JB, and CCHD/DK W1205 to JB. BZ was supported by a postdoctoral fellowship from Biogen and a joint Fonds de recherche Québec – Santé (FRQS)-MSSC fellowship.
Bettina Zierfuss and Agnieszka Buda have contributed equally to this work
Authors and Affiliations
Department of Pathobiology of the Nervous System, Center for Brain Research, Medical University of Vienna, Spitalgasse 4, 1090, Vienna, Austria
Bettina Zierfuss, Agnieszka Buda, Andrea Villoria-González, Maxime Logist, Jure Fabjan, Patricia Parzer, Streggi Vandersteene, Sonja Forss-Petter, Johannes Berger & Isabelle Weinhofer
Department of Neuroscience, Centre de Recherche du CHUM, Université de Montréal, Montréal, H2X 0A9, Canada
Department of Chronic Diseases and Metabolism, Translational Research in GastroIntestinal Disorders, KU Leuven, 3000, Leuven, Belgium
Division of Immune Receptors and T Cell Activation, Institute of Immunology, Center for Pathophysiology, Infectiology and Immunology, Medical University of Vienna, 1090, Vienna, Austria
Claire Battin, Petra Waidhofer-Söllner, Katharina Grabmeier-Pfistershammer & Peter Steinberger
Genetic Metabolic Diseases, Department of Clinical Chemistry, Amsterdam University Medical Center, Amsterdam Neuroscience, Amsterdam Gastroenterology Endocrinology Metabolism, University of Amsterdam, 1105 AZ, Amsterdam, The Netherlands
IW, JB and BZ designed the study. Data were collected by IW, BZ, AB, AVG, ML, PP and SV and analysed by IW, BZ, AB and JB. JF and AB performed the analysis of the whole transcriptome sequencing. CB performed the reporter cell assay and PS contributed with discussion of the data. ESI-MS data were contributed by IMD and SK. PW performed the Luminex assay and KGP contributed with interpretation. IW and BZ wrote the manuscript and BZ created the figures; JB and SFP edited the manuscript. All authors read and approved the final manuscript.
Flow cytometric analysis of CD14+ cell purity. Figure S2. Interactomes integrating dysregulated inflammatory response genes and protein–protein interactions related to cell surface receptors, plasma membrane proteins or G-protein coupled receptors in monocyte-derived macrophages from X-ALD patients versus healthy controls. Figure S3. Viability staining to evaluate cytotoxicity of C26:0 treatment in human primary macrophages. Figure S4. Inhibition of CD36 by SSO co-treatment reduces activation of the JNK pathway in C26:0-exposed macrophages. Figure S5. Pro-inflammatory IL1B, TNF and IL6 cytokine expression is not significantly stimulated by VLCFA C26:0 treatment. Figure S6. C26:0 treatment affects chemokine release by human primary macrophages. Figure S7. Podosome formation in human primary macrophages treated with C26:0. Table S1. Primers used for RT-qPCR analysis
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Zierfuss, B., Buda, A., Villoria-González, A. et al. Saturated very long-chain fatty acids regulate macrophage plasticity and invasiveness.
J Neuroinflammation19, 305 (2022). https://doi.org/10.1186/s12974-022-02664-y